CoraLite® Plus 647 Anti-Human CD79a Rabbit Recombinant Antibody

CD79a Uni-rAb^TM Recombinant Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

241299F3

Cat no : CL647-98072

Synonyms

MB-1 membrane glycoprotein, MB1, MB 1 membrane glycoprotein, MB 1, B-cell antigen receptor complex-associated protein alpha chain

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Validation Data Gallery

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  FC experiment of human PBMCs using CL647-98072

  1x10^6 human PBMCs were intracellularly stained with CoraLite® Plus 488 Anti-Human CD19 (4G7) Mouse IgG2a Recombinant Antibody (CL488-65562, Clone: 4G7), and 5 ul CoraLite® Plus 647 Anti-Human CD79a Rabbit Recombinant Antibody (CL647-98072, Clone:241299F3) or CoraLite® Plus 647 Rabbit IgG Isotype Control Recombinant Antibody (CL647-98136, Clone: 240953C9). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). Lymphocytes were gated.

• 

  FC experiment of human PBMCs using CL647-98072

  1x10^6 human PBMCs were intracellularly stained with 5 ul CoraLite® Plus 647 Anti-Human CD79a Rabbit Recombinant Antibody (CL647-98072, Clone:241299F3) (red) or CoraLite® Plus 647 Rabbit IgG Isotype Control Recombinant Antibody (CL647-98136, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). Lymphocytes were gated.

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Tested Applications

• Positive FC (Intra) detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-98072 targets CD79a in FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant Protein
• Full Name: CD79a molecule, immunoglobulin-associated alpha
• Calculated Molecular Weight: 226 aa, 25 kDa
• GenBank Accession Number: BC113733
• Gene Symbol: CD79A
• Gene ID (NCBI): 973
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD79a, also named as B-cell antigen receptor complex-associated protein alpha chain or MB-1 membrane glycoprotein, is a 226 amino acid protein, which contains one ITAM domain and one Ig-like C2-type (immunoglobulin-like) domain. CD79A is expressed in B cells and localizes in the cell membrane. CD79A is required in cooperation with CD79B for initiation of the signal transduction cascade activated by binding of antigen to the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. CD79A is also required for BCR surface expression and for efficient differentiation of pro- and pre-B-cells.