Anti-Human CD79a Rabbit Recombinant Antibody

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  FC experiment of human PBMCs using 98072-1-RR

  1x10^6 human PBMCs were stained with CoraLite® Plus 647 Anti-Human CD19 (HIB19) (CL647-65110, Clone: HIB19) and 0.25 ug Anti-Human CD79a Rabbit Recombinant Antibody (98072-1-RR, Clone:241299F3), or Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of human PBMCs using 98072-1-RR

  1x10^6 human PBMCs were stained with 0.25 ug Anti-Human CD79a Rabbit Recombinant Antibody (98072-1-RR, Clone:241299F3)(red), or Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 98072-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98072-1-RR against Human CD79a were performed. The affinity constant is 0.151 nM.

CD79a Uni-rAb^TM Recombinant Antibody for FC (Intra)

Cat No. 98072-1-RR

Clone No.241299F3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Synonyms

MB 1, Ig-alpha, IGA, Ig alpha, B-cell antigen receptor complex-associated protein alpha chain

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Tested Applications

• Positive FC (Intra) detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98072-1-RR targets CD79a in FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant Protein
• Full Name: CD79a molecule, immunoglobulin-associated alpha
• Calculated Molecular Weight: 226 aa, 25 kDa
• GenBank Accession Number: BC113733
• Gene Symbol: CD79A
• Gene ID (NCBI): 973
• RRID: AB_3672219
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P11912
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

CD79a, also named as B-cell antigen receptor complex-associated protein alpha chain or MB-1 membrane glycoprotein, is a 226 amino acid protein, which contains one ITAM domain and one Ig-like C2-type (immunoglobulin-like) domain. CD79A is expressed in B cells and localizes in the cell membrane. CD79A is required in cooperation with CD79B for initiation of the signal transduction cascade activated by binding of antigen to the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. CD79A is also required for BCR surface expression and for efficient differentiation of pro- and pre-B-cells.