CoraLite®594-conjugated CD71 Monoclonal antibody

• Featured Product
• KD/KO Validated

CD71 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF/ICC, IF-P

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3C11F11

Cat no : CL594-66180

Synonyms

CD71, p90, sTfR, T9, TFR, TFR1, TFRC, TR, Transferrin receptor protein 1, TRFR

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Validation Data Gallery

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  IF Staining of human breast cancer using CL594-66180

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite®594 CD71 antibody (CL594-66180, Clone: 3C11F11 ) at dilution of 1:100.

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  IF Staining of human breast cancer using CL594-66180

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CoraLite®594 CD71 antibody (CL594-66180, Clone: 3C11F11 ) at dilution of 1:100.

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  IF Staining of MCF-7 using CL594-66180

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite®594 CD71 antibody (CL594-66180, Clone: 3C11F11 ) at dilution of 1:200.

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Tested Applications

• Positive IF-P detected in: human breast cancer tissue
• Positive IF/ICC detected in: MCF-7 cells, human breast cancer tissue

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66180 targets CD71 in IF/ICC, IF-P applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: CD71 fusion protein Ag21612
• Full Name: transferrin receptor (p90, CD71)
• Calculated Molecular Weight: 85 kDa
• Observed Molecular Weight: 90 kDa
• GenBank Accession Number: BC001188
• Gene Symbol: CD71
• Gene ID (NCBI): 7037
• RRID: AB_2919961
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CD71, also known as transferrin receptor protein 1 (TfR1), is a transmembrane glycoprotein composed of two disulfide-linked monomers, each of 90 kDa molecular weight. Each monomer binds one holo-transferrin molecule creating an iron-Tf-TfR complex which enters the cell by endocytosis. CD71 is present on actively proliferating cells and is essential for iron transport into proliferating cells.