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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated CD71 Monoclonal antibody

CD71 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3C11F11

Cat no : CL488-66180

Synonyms

TFR1, TFR, T9, sTfR, p90

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of K-562 cells using CoraLite® Plus 488-conjugated CD71 Monoclonal anti (CL488-66180)

    IF Staining of K-562 using CL488-66180

    Immunofluorescent analysis of (4% PFA) fixed K-562 cells using CoraLite® Plus 488 CD71 antibody (CL488-66180, Clone: 3C11F11 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CoraLite® Plus 488-conjugated CD71 Monoclonal anti (CL488-66180)

    IF Staining of MCF-7 using CL488-66180

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite® Plus 488 CD71 antibody (CL488-66180, Clone: 3C11F11 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of K-562 cells using CoraLite® Plus 488-conjugated CD71 Monoclonal anti (CL488-66180)

    IF Staining of K-562 using CL488-66180

    Immunofluorescent analysis of (4% PFA) fixed K-562 cells using CoraLite® Plus 488 CD71 antibody (CL488-66180, Clone: 3C11F11 ) at dilution of 1:200.

Tested Applications

Positive IF/ICC detected inK-562 cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66180 targets CD71 in IF/ICC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen CD71 fusion protein Ag21612
Full Name transferrin receptor (p90, CD71)
Calculated Molecular Weight 85 kDa
Observed Molecular Weight 90 kDa
GenBank Accession NumberBC001188
Gene Symbol CD71
Gene ID (NCBI) 7037
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD71, also known as transferrin receptor protein 1 (TfR1), is a transmembrane glycoprotein composed of two disulfide-linked monomers, each of 90 kDa molecular weight. Each monomer binds one holo-transferrin molecule creating an iron-Tf-TfR complex which enters the cell by endocytosis. CD71 is present on actively proliferating cells and is essential for iron transport into proliferating cells.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 CD71 antibody CL488-66180Download protocol
Standard Protocols
Click here to view our Standard Protocols