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CD71 Monoclonal antibody

CD71 Monoclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB,IP,IHC,IF,FC,ELISA

Conjugate

Unconjugated

CloneNo.

3C11F11

Cat no : 66180-1-Ig

Synonyms

CD71, p90, sTfR, T9, TFR, TFR1, TFRC, TR, Transferrin receptor protein 1, TRFR



Tested Applications

Positive WB detected inU-251 cells, Raji cells, A549 cells, TF-1 cells, HeLa cells, U2OS cells, A431 cells, HepG2 cells, K-562 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman placenta tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman breast cancer tissue, MCF-7 cells
Positive FC detected inRaji cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66180-1-Ig targets CD71 in WB,IP,IHC,IF,FC,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen CD71 fusion protein Ag21612
Full Name transferrin receptor (p90, CD71)
Calculated Molecular Weight 85 kDa
Observed Molecular Weight 90 kDa
GenBank Accession NumberBC001188
Gene Symbol TFRC
Gene ID (NCBI) 7037
RRIDAB_2881575
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD71, also known as transferrin receptor protein 1 (TfR1), is a transmembrane glycoprotein composed of two disulfide-linked monomers, each of 90 kDa molecular weight. Each monomer binds one holo-transferrin molecule creating an iron-Tf-TfR complex which enters the cell by endocytosis. CD71 is present on actively proliferating cells and is essential for iron transport into proliferating cells.

Protocols

Product Specific Protocols
WB protocol for CD71 antibody 66180-1-IgDownload protocol
IHC protocol for CD71 antibody 66180-1-IgDownload protocol
IF protocol for CD71 antibody 66180-1-IgDownload protocol
IP protocol for CD71 antibody 66180-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Chem Eng J

Functionalized biological metal–organic framework with nanosized coronal structure and hierarchical wrapping pattern for enhanced targeting therapy

Authors - Huafeng Wang
humanWB

Cell Death Dis

Super-enhancer-driven MLX mediates redox balance maintenance via SLC7A11 in osteosarcoma

Authors - Weitang Guo
human,mouseWB

Free Radic Biol Med

Targeting the Na+/K+ ATPase DR-region with DR-Ab improves doxorubicin-induced cardiotoxicity

Authors - Bin Leng
humanWB,Blocking assay

Bioeng Transl Med

Multistage targeting and dual inhibiting strategies based on bioengineered tumor matrix microenvironment-mediated protein nanocages for enhancing cancer biotherapy.

Authors - Fabiao Hu
humanWB

PLoS Genet

SNX-3 mediates retromer-independent tubular endosomal recycling by opposing EEA-1-facilitated trafficking.

Authors - Yangli Tian
humanIF

J Biol Chem

Rab11-mediated post-Golgi transport of the sialyltransferase ST3GAL4 suggests a new mechanism for regulating glycosylation.

Authors - Masato Kitano