CoraLite® Plus 647 Anti-Human CD68 (KP1) Rabbit Recombinant Antibody

CD68 Recombinant Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

KP1

Cat no : CL647-65515

Synonyms

CD68, CD68 molecule, DKFZp686M18236, GP110

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Validation Data Gallery

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  FC experiment of human PBMCs using CL647-65515

  1x10^6 human PBMCs were intracellularly stained with 5 ul CoraLite® Plus 647 Anti-Human CD68 Rabbit Recombinant Antibody (CL647-65515, Clone:KP1) or CoraLite® Plus 647-conjugated Rabbit IgG control PolyAb (CL647-30000). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Cells were treated with FC Receptor Block prior to staining.

• 

  FC experiment of human PBMCs using CL647-65515

  1x10^6 human PBMCs were intracellularly stained with 5 ul CoraLite® Plus 647 Anti-Human CD68 Rabbit Recombinant Antibody (CL647-65515, Clone:KP1) or CoraLite® Plus 647-conjugated Rabbit IgG control PolyAb (CL647-30000). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Cells were treated with FC Receptor Block prior to staining.

• 

  FC experiment of human PBMCs using CL647-65515

  1x10^6 human PBMCs were intracellularly stained with 5 ul CoraLite® Plus 647 Anti-Human CD68 Rabbit Recombinant Antibody (CL647-65515, Clone:KP1) or 5 ul CoraLite® Plus 647 Anti-Human CD68 Mouse Monoclonal Antibody (CL647-65202, Clone:KP1). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Cells were treated with FC Receptor Block prior to staining.

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-65515 targets CD68 in FC (Intra) applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Lysosomal fraction of human alveolar macrophages
• Full Name: CD68 molecule
• Calculated Molecular Weight: 37 kDa
• GenBank Accession Number: BC015557
• Gene Symbol: CD68
• Gene ID (NCBI): 968
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD68 is a type I transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages.