CoraLite®594-conjugated CD68 Polyclonal antibody

CD68 Polyclonal Antibody for FC (Intra), IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

Publications

1

Cat no : CL594-25747

Synonyms

CD68, CD68 molecule, DKFZp686M18236, GP110, Macrosialin, SCARD1

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Validation Data Gallery

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  IF Staining of human colon using CL594-25747

  Immunofluorescent analysis of (4% PFA) fixed human colon tissue using CoraLite®594 CD68 antibody (CL594-25747) at dilution of 1:200, Cytokeratin 20 antibody (60183-1-Ig, Clone: 4D10A4, Magenta), Collagen Type III (N-terminal) antibody (22734-1-AP, green).

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  IF Staining of human tonsillitis using CL594-25747

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594 CD68 antibody (CL594-25747) at dilution of 1:200.

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  IF Staining of human tonsillitis using CL594-25747

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594 CD68 antibody (CL594-25747) at dilution of 1:200.

• 

  IF Staining of human colon using CL594-25747

  Immunofluorescent analysis of (4% PFA) fixed human colon tissue using CoraLite®594 CD68 antibody (CL594-25747) at dilution of 1:50, CoraLite®488 Cytokeratin 20 antibody (CL488-60183, Clone: 4D10A4, green).

• 

  IF Staining of human colon using CL594-25747

  Immunofluorescent analysis of (4% PFA) fixed human colon tissue using CoraLite®594 CD68 antibody (CL594-25747) at dilution of 1:50, CoraLite®488 Cytokeratin 20 antibody (CL488-60183, Clone: 4D10A4, green).

• 

  FC experiment of RAW 264.7 using CL594-25747

  1X10^6 RAW 264.7 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human CD68 (CL594-25747) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF detected in: human colon tissue, human tonsillitis tissue
• Positive FC detected in: RAW 264.7 cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

The immunogen of CL594-25747 is CD68 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CD68 fusion protein Ag22815
• Full Name: CD68 molecule
• Calculated Molecular Weight: 37 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC015557
• Gene Symbol: CD68
• Gene ID (NCBI): 968
• RRID: AB_2919875
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD68 is a type I transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages. The apparent molecular weight of CD68 is larger than calculated molecular weight due to post-translation modification.

Publications

Species	Application	Title
mouse	IF	Front Mol Biosci; Blockage of ERCC6 Alleviates Spinal Cord Injury Through Weakening Apoptosis, Inflammation, Senescence, and Oxidative Stress.; Authors - Peng Zou

Related Products

Flow Cytometry Perm Buffer (10X)

PF00011-C