Cytokeratin 20 Recombinant antibody
Cytokeratin 20 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat, pig
Applications
WB, IHC, IF/ICC, FC (Intra), ELISA
Conjugate
Unconjugated
CloneNo.
2H10
Cat no : 82428-1-RR
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HT-29 cells, COLO 320 cells, pig colon tissue, mouse colon tissue, rat colon tissue |
| Positive IHC detected in | human colon cancer tissue, human appendicitis tissue, human colon tissue, human urothelial carcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HT-29 cells |
| Positive FC (Intra) detected in | HT-29 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:3000-1:12000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
82428-1-RR targets Cytokeratin 20 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Reactivity | human, mouse, rat, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | Cytokeratin 20 fusion protein Ag11308 |
| Full Name | keratin 20 |
| Calculated Molecular Weight | 424 aa, 48 kDa |
| Observed Molecular Weight | 46 kDa |
| GenBank Accession Number | BC031559 |
| Gene Symbol | Cytokeratin 20 |
| Gene ID (NCBI) | 54474 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 20 is a type I cytokeratin. It is a major cellular protein of mature enterocytes and goblet cells and is specifically found in the gastric and intestinal mucosa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Cytokeratin 20 antibody 82428-1-RR | Download protocol |
| IHC protocol for Cytokeratin 20 antibody 82428-1-RR | Download protocol |
| IF protocol for Cytokeratin 20 antibody 82428-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
