FITC Plus Anti-Human CD58 (TS2/9) Mouse IgG1 Recombinant Antibody

CD58 Recombinant Antibody for FC

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

FC

Conjugate

FITC Plus Fluorescent Dye

CloneNo.

TS2/9

Cat no : FITC-65517

Synonyms

Ag3, CD58, CD58 molecule, FLJ23181, FLJ43722, LFA 3, LFA3, Surface glycoprotein LFA 3

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Validation Data Gallery

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  FC experiment of human PBMCs using FITC-65517

  1x10^6 human PBMCs were surface stained with CL750 Anti-Human CD14 and 5 ul FITC Plus Anti-Human CD58 Mouse Recombinant Antibody (FITC-65517, Clone:TS2/9) or FITC Plus Mouse IgG1 Isotype Control (FITC-65124, Clone: MOPC-21). Cells were not fixed. Cells were treated with FC Receptor Block prior to staining. Monocytes were gated.

• 

  FC experiment of human PBMCs using FITC-65517

  1x10^6 human PBMCs were surface stained with 5 ul FITC Plus Anti-Human CD58 Mouse Recombinant Antibody (FITC-65517, Clone:TS2/9) or FITC Plus Mouse IgG1 Isotype Control (FITC-65124, Clone: MOPC-21). Cells were not fixed. Cells were treated with FC Receptor Block prior to staining. Monocytes were gated.

• 

  FC experiment of human PBMCs using FITC-65517

  1x10^6 human PBMCs were surface stained with CL750 Anti-Human CD14 and 5 ul FITC Plus Anti-Human CD58 Mouse Recombinant Antibody (FITC-65517, Clone:TS2/9) or 5 ul FITC Plus Anti-Human CD58 Mouse Monoclonal Antibody (FITC-65193, Clone:TS2/9). Cells were not fixed. Cells were treated with FC Receptor Block prior to staining. Monocytes were gated.

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

FITC-65517 targets CD58 in FC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Recombinant
• Type: Antibody
• Immunogen: Human cytolytic T cells
• Full Name: CD58 molecule
• Calculated Molecular Weight: 28 kDa
• GenBank Accession Number: BC005930
• Gene Symbol: CD58
• Gene ID (NCBI): 965
• Conjugate: FITC Plus Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 495 nm / 524 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD58, also known as lymphocyte function-associated antigen 3 (LFA-3), is a heavily glycosylated protein of 55-70 kDa that is expressed on a broad range of hematopoietic and non-hematopoietic cells. CD58 is involved in immune recognition of tumor cells via binding of the CD2 receptor expressed on cytotoxic T cells.