Anti-Human CD58 (TS2/9) Mouse IgG1 Recombinant Antibody

CD58 Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

FC

Conjugate

Unconjugated

CloneNo.

TS2/9

Cat no : 65517-1-MR

Synonyms

Ag3, CD58, CD58 molecule, FLJ23181, FLJ43722, LFA 3, LFA3, Surface glycoprotein LFA 3

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Validation Data Gallery

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  FC experiment of human PBMCs using 65517-1-MR

  1x10^6 human PBMCs were surface stained with 0.1 ug Anti-Human CD58 Mouse Recombinant Antibody (65517-1-MR, Clone:TS2/9) or Mouse IgG1 Isotype Control, and CoraLite®647-Conjugated Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were then stained with FITC-conjugated Anti-Human CD14. Cells were not fixed. Cells were treated with FC Receptor Block prior to staining. Monocytes were gated.

• 

  FC experiment of human PBMCs using 65517-1-MR

  1x10^6 human PBMCs were surface stained with 0.1 ug Anti-Human CD58 Mouse Recombinant Antibody (65517-1-MR, Clone:TS2/9) or Mouse IgG1 Isotype Control, and CoraLite®647-Conjugated Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were not fixed. Cells were treated with FC Receptor Block prior to staining. Monocytes were gated.

• 

  FC experiment of human PBMCs using 65517-1-MR

  1x10^6 human PBMCs were surface stained with 0.1 ug Anti-Human CD58 Mouse Recombinant Antibody (65517-1-MR, Clone:TS2/9) (red), or 0.1 ug Anti-Human CD58 Mouse Monoclonal Antibody (CloneTS2/9) (65193-1-Ig in green or from another vendor in pink), or Mouse IgG1 Isotype Control (blue), and CoraLite®647-Conjugated Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were not fixed. Cells were treated with FC Receptor Block prior to staining. Monocytes were gated.

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65517-1-MR targets CD58 in FC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Recombinant
• Type: Antibody
• Immunogen: Human cytolytic T cells
• Full Name: CD58 molecule
• Calculated Molecular Weight: 28 kDa
• GenBank Accession Number: BC005930
• Gene Symbol: CD58
• Gene ID (NCBI): 965
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

CD58, also known as lymphocyte function-associated antigen 3 (LFA-3), is a heavily glycosylated protein of 55-70 kDa that is expressed on a broad range of hematopoietic and non-hematopoietic cells. CD58 is involved in immune recognition of tumor cells via binding of the CD2 receptor expressed on cytotoxic T cells.