PE Anti-Human CD45 Rabbit Recombinant Antibody

CD45 Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

PE Fluorescent Dye

CloneNo.

241670E10

Cat no : PE-98117

Synonyms

L CA, GP180, EC:3.1.3.48, CD45R, B220

---

Validation Data Gallery

• 

  FC experiment of human peripheral blood leukocytes using PE-98117

  1x10^6 human peripheral blood leukocytes were surface stained with 5 ul PE Anti-Human CD45 Rabbit Recombinant Antibody (PE-98117, Clone: 241670E10), or Isotype Control (PE-98136, Clone:240953C9). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human peripheral blood leukocytes using PE-98117

  1x10^6 human peripheral blood leukocytes were surface stained with 5 ul PE Anti-Human CD45 Rabbit Recombinant Antibody (PE-98117, Clone: 241670E10) (red), or Isotype Control (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

---

Tested Applications

• Positive FC detected in: human peripheral blood leukocyte

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of PE-98117 is CD45 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: protein tyrosine phosphatase, receptor type, C
• Gene Symbol: CD45
• Gene ID (NCBI): 5788
• Conjugate: PE Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097).