APC-Cyanine7 Anti-Human CD45 Rabbit Recombinant Antibody

CD45 Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

APC-Cyanine7 Fluorescent Dye

CloneNo.

241670E10

Cat no : AY7-98117

Synonyms

LCA, L CA, GP180, EC:3.1.3.48, B220

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Validation Data Gallery

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  FC experiment of human peripheral blood leukocytes using AY7-98117

  1x10^6 human peripheral blood leukocytes were unstained or surface stained with 5 ul APC-Cyanine7 Anti-Human CD45 Rabbit Recombinant Antibody (AY7-98117, Clone: 241670E10). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human peripheral blood leukocytes using AY7-98117

  1x10^6 human peripheral blood leukocytes were unstained (blue) or surface stained with 5 ul APC-Cyanine7 Anti-Human CD45 Rabbit Recombinant Antibody (AY7-98117, Clone: 241670E10) (red). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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Tested Applications

• Positive FC detected in: human peripheral blood leukocytes

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of AY7-98117 is CD45 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: protein tyrosine phosphatase, receptor type, C
• Gene Symbol: CD45
• Gene ID (NCBI): 5788
• Conjugate: APC-Cyanine7 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 650 nm / 778 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097).