CoraLite® Plus 750 Anti-Human CD45 Rabbit Recombinant Antibody

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  FC experiment of human peripheral blood leukocytes using CL750-98117

  1x10^6 human peripheral blood leukocytes were surface stained with 5 ul CoraLite® Plus 750 Anti-Human CD45 Rabbit Recombinant Antibody (CL750-98117, Clone: 241670E10) or CoraLite® Plus 750 Rabbit IgG Isotype Control Recombinant Antibody (CL750-98136, Clone: 240953C9). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human peripheral blood leukocytes using CL750-98117

  1x10^6 human peripheral blood leukocytes were surface stained with 5 ul CoraLite® Plus 750 Anti-Human CD45 Rabbit Recombinant Antibody (CL750-98117, Clone: 241670E10) red) or CoraLite® Plus 750 Rabbit IgG Isotype Control Recombinant Antibody (CL750-98136, Clone: 240953C9) (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

CD45 Uni-rAb^TM Recombinant Antibody for FC

Cat No. CL750-98117

Clone No.241670E10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

L CA, GP180, EC:3.1.3.48, CD45R, B220

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Tested Applications

• Positive FC detected in: FC receptor blocked human peripheral blood leukocytes

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CL750-98117 targets CD45 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: protein tyrosine phosphatase, receptor type, C
• Gene Symbol: CD45
• Gene ID (NCBI): 5788
• ENSEMBL Gene ID: ENSG00000081237
• RRID: AB_3673806
• Conjugate: CoraLite® Plus 750 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 755 nm / 780 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097).