CoraLite® Plus 594 Anti-Human CD33 (WM53)

CD33 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human

Applications

FC

Conjugate

CoraLite® Plus 594 Fluorescent Dye

CloneNo.

WM53

Cat no : CL594-65272

Synonyms

SIGLEC3, SIGLEC 3, Sialic acid-binding Ig-like lectin 3, p67, gp67

---

Validation Data Gallery

• 

  FC experiment of human PBMCs using CL594-65272

  1x10^6 FC receptor blocked human PBMCs were surface stained with 5 ul CoraLite® Plus 594 Anti-Human CD33 (CL594-65272, Clone: WM53) (red) or CoraLite®594 Mouse IgG1 Isotype Control (blue). Cells were not fixed. Monocytes were gated.

• 

  FC experiment of human PBMCs using CL594-65272

  1x10^6 FC receptor blocked human PBMCs were surface stained with 5 ul CoraLite® Plus 594 Anti-Human CD33 (CL594-65272, Clone: WM53) or CoraLite®594 Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes and monocytes were gated.

---

Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-65272 targets CD33 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: N/A
• Full Name: CD33 molecule
• Calculated Molecular Weight: 364 aa, 40 kDa
• GenBank Accession Number: BC028152
• Gene Symbol: CD33
• Gene ID (NCBI): 945
• Conjugate: CoraLite® Plus 594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 594 nm / 615 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light.

Background Information

CD33, also referred to as Siglec-3, is a 67-kDa glycosylated transmembrane protein of sialic acid-binding immunoglobulin-like lactic (siglec) family. CD33 is expressed on monocytes, myeloid progenitors, granulocytes, mast cells, some T cells. It may mediate cell-to-cell adhesion and act as a receptor that inhibits the proliferation of normal and leukemic myeloid cells.