CoraLite® Plus 405 Anti-Human CD33 (WM53)

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  FC experiment of human PBMCs using CL405-65272

  1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 405 Anti-Human CD33 (CL405-65272, Clone: WM53) (red) or unstained. Cells were not fixed. Monocytes were gated.

• 

  FC experiment of human PBMCs using CL405-65272

  1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 405 Anti-Human CD33 (CL405-65272, Clone: WM53) or unstained. Cells were not fixed. Monocytes were gated.

CD33 Monoclonal Antibody for FC

Cat No. CL405-65272

Clone No.WM53

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

FC

Synonyms

CD33, CD33 molecule, FLJ00391, gp67, p67, SIGLEC 3, SIGLEC3

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 5 ul per 10^6 cells in 100 μl suspension

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.

Sample-dependent, Check data in validation data gallery.

Product Information

CL405-65272 targets CD33 in FC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  N/A

• Full Name: CD33 molecule
• Calculated Molecular Weight: 364 aa, 40 kDa
• GenBank Accession Number: BC028152
• Gene Symbol: CD33
• Gene ID (NCBI): 945
• RRID: AB_3083854
• Conjugate: CoraLite® Plus 405 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 399 nm / 422 nm
• Form: Liquid
• Purification Method: Affinity purification
• UNIPROT ID: P20138
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD33, also referred to as Siglec-3, is a 67-kDa glycosylated transmembrane protein of sialic acid-binding immunoglobulin-like lactic (siglec) family. CD33 is expressed on monocytes, myeloid progenitors, granulocytes, mast cells, some T cells. It may mediate cell-to-cell adhesion and act as a receptor that inhibits the proliferation of normal and leukemic myeloid cells.