CoraLite® Plus 647 Anti-Mouse CD324 (E-cadherin) (DECMA-1)
CD324 (E-cadherin) Monoclonal Antibody for FC
Host / Isotype
Rat / IgG1, kappa
Reactivity
Mouse, Human, Canine and More (1)
Applications
FC
Conjugate
CoraLite® Plus 647 Fluorescent Dye
CloneNo.
DECMA-1
Cat no : CL647-65241
Synonyms
Validation Data Gallery
Tested Applications
| Positive FC detected in | MDCK cells |
Recommended dilution
| Application | Dilution |
|---|---|
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL647-65241 targets CD324 (E-cadherin) in FC applications and shows reactivity with Mouse, Human, Canine samples.
| Tested Reactivity | Mouse, Human, Canine |
| Cited Reactivity | mouse |
| Host / Isotype | Rat / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Mouse embryonal carcinoma cell line PCC4 Aza RI |
| Full Name | cadherin 1 |
| GenBank Accession Number | BC098501 |
| Gene Symbol | E-cadherin |
| Gene ID (NCBI) | 12550 |
| Conjugate | CoraLite® Plus 647 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 654 nm / 674 nm |
| Form | Liquid |
| Purification Method | N/A |
| Storage Buffer | PBS with 0.09% sodium azide. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. It has been regarded as a marker for spermatogonial stem cells in mice(PMID:23509752). E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 647 CD324 (E-cadherin) antibody CL647-65241 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Cell surface patching via CXCR4-targeted nanothreads for cancer metastasis inhibition |
