PE Anti-Mouse CD324 (E-cadherin) (DECMA-1)

CD324 (E-cadherin) Monoclonal Antibody for FC

Host / Isotype

Rat / IgG1, kappa

Reactivity

Canine, Mouse, Human

Applications

FC

Conjugate

PE Fluorescent Dye

CloneNo.

DECMA-1

Cat no : PE-65241

Synonyms

AA960649, cadherin 1, Cdh1, Ecad, L CAM, Um, UVO

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Validation Data Gallery

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  FC experiment of MDCK using PE-65241

  1x10^6 MDCK cells were surface stained with 0.2 ug PE Anti-Mouse CD324 (E-cadherin) (DECMA-1) (PE-65241, Clone:DECMA-1), or 0.2 ug PE Rat IgG1 Isotype Control (HRPN) (PE-65212, Clone: HRPN). Cells were not fixed.

• 

  FC experiment of MDCK using PE-65241

  1x10^6 MDCK cells were surface stained with 0.2 ug PE Anti-Mouse CD324 (E-cadherin) (DECMA-1) (PE-65241, Clone:DECMA-1) (red), or 0.2 ug PE Rat IgG1 Isotype Control (HRPN) (PE-65212, Clone: HRPN) (blue). Cells were not fixed.

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Tested Applications

• Positive FC detected in: MDCK cells

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

PE-65241 targets CD324 (E-cadherin) in FC applications and shows reactivity with Canine, Mouse, Human samples.

• Tested Reactivity: Canine, Mouse, Human
• Host / Isotype: Rat / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Mouse embryonal carcinoma cell line PCC4 Aza RI
• Full Name: cadherin 1
• GenBank Accession Number: BC098501
• Gene Symbol: Cdh1
• Gene ID (NCBI): 12550
• Conjugate: PE Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Purification Method: N/A
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. It has been regarded as a marker for spermatogonial stem cells in mice(PMID:23509752). E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness.