Anti-Mouse CD324 (E-cadherin) (DECMA-1)

CD324 (E-cadherin) Monoclonal Antibody for IF-P, FC

Host / Isotype

Rat / IgG1, kappa

Reactivity

human, mouse, canine

Applications

IF-P, FC

Conjugate

Unconjugated

CloneNo.

DECMA-1

Cat no : 65241-1-Ig

Synonyms

E-cadherin, Um, L CAM, Ecad, Cdh1

---

Validation Data Gallery

• 

  IF Staining of mouse colon using 65241-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using CD324 (E-cadherin) antibody (65241-1-Ig, Clone: DECMA-1 ) at dilution of 1:1250 and Rhodamine (TRITC)-conjugated Goat Anti-Rat IgG(H+L).

• 

  IF Staining of mouse colon using 65241-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using CD324 (E-cadherin) antibody (65241-1-Ig, Clone: DECMA-1 ) at dilution of 1:1250 and Rhodamine (TRITC)-conjugated Goat Anti-Rat IgG(H+L).

• 

  IF Staining of mouse colon using 65241-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse colon tissue using CD324 (E-cadherin) antibody (65241-1-Ig, Clone: DECMA-1 ) at dilution of 1:100 and Fluorescein (FITC)-conjugated Goat Anti-Rat IgG(H+L) SA00003-11. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of MDCK using 65241-1-Ig

  1X10^6 MDCK cells were surface stained with 0.2 ug Anti-Mouse CD324 (E-cadherin) (65241-1-Ig, Clone:DECMA-1) and APC-conjugated Goat Anti-Rat IgG at dilution 1:1000. Cells were not fixed.

---

Tested Applications

• Positive IF-P detected in: mouse colon tissue
• Positive FC detected in: MDCK cells

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:625-1:2500

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

65241-1-Ig targets CD324 (E-cadherin) in IF-P, FC applications and shows reactivity with human, mouse, canine samples.

• Tested Reactivity: human, mouse, canine
• Host / Isotype: Rat / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Mouse embryonal carcinoma cell line PCC4 Aza RI
• Full Name: cadherin 1
• GenBank Accession Number: BC098501
• Gene Symbol: E-cadherin
• Gene ID (NCBI): 12550
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. It has been regarded as a marker for spermatogonial stem cells in mice(PMID:23509752). E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness.