Anti-Mouse E-cadherin (DECMA-1)

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  IF Staining of mouse colon using 65241-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using CD324 (E-cadherin) antibody (65241-1-Ig, Clone: DECMA-1 ) at dilution of 1:1250 and Rhodamine (TRITC)-conjugated Goat Anti-Rat IgG(H+L).

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  IF Staining of mouse colon using 65241-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using CD324 (E-cadherin) antibody (65241-1-Ig, Clone: DECMA-1 ) at dilution of 1:1250 and Rhodamine (TRITC)-conjugated Goat Anti-Rat IgG(H+L).

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  IF Staining of mouse colon using 65241-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse colon tissue using CD324 (E-cadherin) antibody (65241-1-Ig, Clone: DECMA-1 ) at dilution of 1:100 and Fluorescein (FITC)-conjugated Goat Anti-Rat IgG(H+L) SA00003-11. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of MDCK using 65241-1-Ig

  1X10^6 MDCK cells were surface stained with 0.2 ug Anti-Mouse CD324 (E-cadherin) (65241-1-Ig, Clone:DECMA-1) and APC-conjugated Goat Anti-Rat IgG at dilution 1:1000. Cells were not fixed.

E-cadherin Monoclonal Antibody for IF-P, FC

Cat No. 65241-1-Ig

Clone No.DECMA-1

Host / Isotype

Rat / IgG1, kappa

Reactivity

human, mouse, canine

Applications

IF-P, FC

Synonyms

CD324 (E-cadherin), cadherin 1, Cdh1, Ecad, L CAM

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Tested Applications

• Positive IF-P detected in: mouse colon tissue
• Positive FC detected in: MDCK cells

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:625-1:2500
• Flow Cytometry (FC): FC : 0.2 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

65241-1-Ig targets E-cadherin in IF-P, FC applications and shows reactivity with human, mouse, canine samples.

• Tested Reactivity: human, mouse, canine
• Host / Isotype: Rat / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  Mouse embryonal carcinoma cell line PCC4 Aza RI

• Full Name: cadherin 1
• GenBank Accession Number: BC098501
• Gene Symbol: E-cadherin
• Gene ID (NCBI): 12550
• RRID: AB_2935150
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Affinity purification
• UNIPROT ID: P09803
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. It has been regarded as a marker for spermatogonial stem cells in mice(PMID:23509752). E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness.