CoraLite® Plus 488 Anti-Mouse CD3ε (145-2C11)

CD3ε Monoclonal Antibody for FC

Host / Isotype

Hamster / IgG

Reactivity

Mouse

Applications

FC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

145-2C11

Cat no : CL488-65060

Synonyms

AI504783, CD3, CD3e, CD3epsilon, CD3ε, T3e



Tested Applications

Positive FC detected inmouse splenocytes

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-65060 targets CD3ε in FC applications and shows reactivity with Mouse samples.

Tested Reactivity Mouse
Host / Isotype Hamster / IgG
Class Monoclonal
Type Antibody
Immunogen H-2Kb-specific murine cytotoxic T-lymphocyte (CTL) clone BM10-37
Full Name CD3 antigen, epsilon polypeptide
GenBank Accession NumberBC098236
Gene Symbol CD3ε
Gene ID (NCBI) 12501
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 CD3ε antibody CL488-65060Download protocol
Standard Protocols
Click here to view our Standard Protocols