Anti-Mouse CD3ε (145-2C11)

CD3ε Monoclonal Antibody for IF/ICC, FC

Host / Isotype

Hamster / IgG

Reactivity

Mouse

Applications

IF/ICC, FC

Conjugate

Unconjugated

CloneNo.

145-2C11

Reviews (1)

Cat no : 65060-1-Ig

Synonyms

AI504783, CD3, CD3e, CD3epsilon, CD3ε, T3e

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Validation Data Gallery

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  IF Staining of mouse splenocytes using 65060-1-Ig

  Immunofluorescent analysis of mouse splenocytes using CD3ε antibody (65060-1-Ig, Clone: 145-2C11 ) at dilution of 1:500 and FITC Goat anti-Hamster(Armenian) IgG.

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  FC experiment of C57BL/6 mouse splenocytes using 65060-1-Ig

  1X10^6 C57BL/6 mouse splenocytes were surface co-stained with CoraLite® Plus 647 Anti-Mouse CD4 (GK1.5) and 0.5 ug Anti-Mouse CD3ε (65060-1-Ig, Clone:145-2C11) or 0.5 ug Armenian Hamster IgG Isotype Control, and FITC anti-Armenian Hamster IgG antibody at dilution 1:100. Cells were not fixed.

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Tested Applications

• Positive IF/ICC detected in: mouse splenocytes
• Positive FC detected in: C57BL/6 mouse splenocytes

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

65060-1-Ig targets CD3ε in IF/ICC, FC applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Host / Isotype: Hamster / IgG
• Class: Monoclonal
• Type: Antibody
• Immunogen: H-2Kb-specific murine cytotoxic T-lymphocyte (CTL) clone BM10-37
• Full Name: CD3 antigen, epsilon polypeptide
• GenBank Accession Number: BC098236
• Gene Symbol: CD3ε
• Gene ID (NCBI): 12501
• RRID: AB_2918368
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Helena (Verified Customer) (07-22-2022)

Not many t cells to detect but they were observable in this tissue section.

• Applications: Immunohistochemistry, Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mouse brain tissue post stroke

CD3ε Antibody Immunohistochemistry,Immunofluorescence validation (1:1000 dilution) in mouse brain tissue post stroke (Cat no:65060-1-Ig)