CoraLite® Plus 647 Anti-Mouse CD3ε (145-2C11)

CD3ε Monoclonal Antibody for FC

Host / Isotype

Hamster / IgG

Reactivity

mouse

Applications

FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

145-2C11

Cat no : CL647-65060

Synonyms

Cd3e, T3e, CD3epsilon, CD3, AI504783

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Validation Data Gallery

• 

  FC experiment of mouse splenocytes using CL647-65060

  1x10^6 mouse splenocytes were surface stained with 0.25 ug CoraLite® Plus 647 Anti-Mouse CD3ε (145-2C11) (CL647-65060, Clone:145-2C11) or 0.25 ug Isotype Control, and 0.25 ug PE Anti-Mouse CD4 (GK1.5) (PE-65104, Clone: GK1.5). Cells were not fixed.

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Tested Applications

• Positive FC detected in: mouse splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-65060 targets CD3ε in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Hamster / IgG
• Class: Monoclonal
• Type: Antibody
• Immunogen: H-2Kb-specific murine cytotoxic T-lymphocyte (CTL) clone BM10-37
• Full Name: CD3 antigen, epsilon polypeptide
• GenBank Accession Number: BC098236
• Gene Symbol: CD3ε
• Gene ID (NCBI): 12501
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).