CoraLite®594-conjugated CD23 Monoclonal antibody

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  IF Staining of human tonsillitis using CL594-60208-2

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using CoraLite®594 CD23 antibody (CL594-60208-2, Clone: 4C7C9 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CD23 Monoclonal Antibody for IF-P

Cat No. CL594-60208-2

Clone No.4C7C9

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

IF-P

Synonyms

C-type lectin domain family 4 member J, CLEC4J, CD23A, BLAST-2, BLAST 2

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Tested Applications

• Positive IF-P detected in: human tonsillitis tissue

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-60208-2 targets CD23 in IF-P applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: CD23 fusion protein Ag0425
• Full Name: Fc fragment of IgE, low affinity II, receptor for (CD23)
• Calculated Molecular Weight: 321 aa, 36 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC064417
• Gene Symbol: CD23
• Gene ID (NCBI): 2208
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P06734
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CD23, also known as low affinity immunoglobulin epsilon Fc receptor, is a transmembrane glycoprotein present on a subpopulation of B lymphocytes in germinal centres, EBV-transformed B-lymphoblastoid cell lines, follicular dendritic cells, and a subpopulation of peripheral blood cells. CD23 has essential roles in the regulation of IgE production and in the differentiation of B-cells.