CD23 Monoclonal antibody

CD23 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

4C7C9

Cat no : 60208-2-Ig

Synonyms

BLAST 2, CD23, CD23A, CLEC4J, Fc epsilon RII, FCE2, FCER2, IGEBF, Lymphocyte IgE receptor

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Validation Data Gallery

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  WB analysis of Raji using 60208-2-Ig

  Raji cells were subjected to SDS PAGE followed by western blot with 60208-2-Ig (CD23 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 60208-2-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60208-2-Ig (CD23 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 60208-2-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60208-2-Ig (CD23 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 60208-2-Ig

  Immunohistochemical analysis of paraffin-embedded human diffuse small B-cell lymphoma using 60208-2-Ig(CD23 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lymphoma using 60208-2-Ig

  Immunohistochemical analysis of paraffin-embedded human diffuse small B-cell lymphoma using 60208-2-Ig(CD23 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of human tonsillitis using 60208-2-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 60208-2-Ig (CD23 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human tonsillitis using 60208-2-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 60208-2-Ig (CD23 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: Raji cells
• Positive IHC detected in: human tonsillitis tissue, human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human tonsillitis tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

60208-2-Ig targets CD23 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: CD23 fusion protein Ag0425
• Full Name: Fc fragment of IgE, low affinity II, receptor for (CD23)
• Calculated Molecular Weight: 321 aa, 36 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC064417
• Gene Symbol: CD23
• Gene ID (NCBI): 2208
• RRID: AB_11232221
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD23, also known as low affinity immunoglobulin epsilon Fc receptor, is a transmembrane glycoprotein present on a subpopulation of B lymphocytes in germinal centres, EBV-transformed B-lymphoblastoid cell lines, follicular dendritic cells, and a subpopulation of peripheral blood cells. CD23 has essential roles in the regulation of IgE production and in the differentiation of B-cells.