CoraLite® Plus 488-conjugated CD23 Monoclonal antibody

CD23 Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

4C7C9

Cat no : CL488-60208

Synonyms

BLAST 2, CD23, CD23A, CLEC4J, Fc epsilon RII, FCE2, FCER2, IGEBF, Lymphocyte IgE receptor

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Validation Data Gallery

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  IF Staining of human tonsillitis using CL488-60208

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CL488-60208 (CD23 antibody) at dilution of 1:50 .

• 

  IF Staining of human tonsillitis using CL488-60208

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CL488-60208 (CD23 antibody) at dilution of 1:50 .

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Tested Applications

• Positive IF-P detected in: human tonsillitis tissue

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL488-60208 is CD23 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: CD23 fusion protein Ag0425
• Full Name: Fc fragment of IgE, low affinity II, receptor for (CD23)
• Calculated Molecular Weight: 321 aa, 36 kDa
• GenBank Accession Number: BC064417
• Gene Symbol: CD23
• Gene ID (NCBI): 2208
• RRID: AB_2883121
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CD23, also known as low affinity immunoglobulin epsilon Fc receptor, is a transmembrane glycoprotein present on a subpopulation of B lymphocytes in germinal centres, EBV-transformed B-lymphoblastoid cell lines, follicular dendritic cells, and a subpopulation of peripheral blood cells. CD23 has essential roles in the regulation of IgE production and in the differentiation of B-cells. And the antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.