1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD23 Rabbit Recombinant Antibody (98088-1-RR, Clone:241270C6) or Isotype Control and PE-conjugated Goat Anti-Rabbit IgG. Cell were then stained with CoraLite® Plus 647 Anti-Human CD19 (4G7). Cells were not fixed. Lymphocytes were gated.
1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD23 Rabbit Recombinant Antibody (98088-1-RR, Clone:241270C6) or Isotype Control and PE-conjugated Goat Anti-Rabbit IgG. Cell were then stained with CoraLite® Plus 647 Anti-Human CD19 (4G7). Cells were not fixed. Lymphocytes were gated.
FC experiment of human PBMCs using 98088-1-RR
1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD23 Rabbit Recombinant Antibody (98088-1-RR, Clone:241270C6) (red) or Isotype Control (blue) and PE-conjugated Goat Anti-Rabbit IgG. Cells were not fixed. Lymphocytes were gated.
1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD23 Rabbit Recombinant Antibody (98088-1-RR, Clone:241270C6) (red) or Isotype Control (blue) and PE-conjugated Goat Anti-Rabbit IgG. Cells were not fixed. Lymphocytes were gated.
FC experiment of human PBMCs using 98088-1-RR
1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD23 Rabbit Recombinant Antibody (98088-1-RR, Clone:241270C6) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), and 0.25 ug Isotype Control, and 0.25 ug CoraLite® Plus 647 Anti-Human CD19 (4G7) Mouse IgG2a Recombinant Antibody (CL647-65562, Clone: 4G7). Cells were not fixed.
1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD23 Rabbit Recombinant Antibody (98088-1-RR, Clone:241270C6) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), and 0.25 ug Isotype Control, and 0.25 ug CoraLite® Plus 647 Anti-Human CD19 (4G7) Mouse IgG2a Recombinant Antibody (CL647-65562, Clone: 4G7). Cells were not fixed.
Affinity and Kinetic Characterization of 98088-1-RR
Biolayer interferometry (BLl) kinetic assays of 98088-1-RR against Human CD23 were performed. The affinity constant is below 1 pM.
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
98088-1-RR targets CD23 in FC applications and shows reactivity with human samples.
Store at 2 - 8°C. Stable for one year after shipment.
Background Information
CD23, also known as low affinity immunoglobulin epsilon Fc receptor, is a transmembrane glycoprotein present on a subpopulation of B lymphocytes in germinal centres, EBV-transformed B-lymphoblastoid cell lines, follicular dendritic cells, and a subpopulation of peripheral blood cells. CD23 has essential roles in the regulation of IgE production and in the differentiation of B-cells.
1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD23 Rabbit Recombinant Antibody (98088-1-RR, Clone:241270C6) or Isotype Control and PE-conjugated Goat Anti-Rabbit IgG. Cell were then stained with CoraLite® Plus 647 Anti-Human CD19 (4G7). Cells were not fixed. Lymphocytes were gated.
FC experiment of human PBMCs using 98088-1-RR
1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD23 Rabbit Recombinant Antibody (98088-1-RR, Clone:241270C6) (red) or Isotype Control (blue) and PE-conjugated Goat Anti-Rabbit IgG. Cells were not fixed. Lymphocytes were gated.
FC experiment of human PBMCs using 98088-1-RR
1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD23 Rabbit Recombinant Antibody (98088-1-RR, Clone:241270C6) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), and 0.25 ug Isotype Control, and 0.25 ug CoraLite® Plus 647 Anti-Human CD19 (4G7) Mouse IgG2a Recombinant Antibody (CL647-65562, Clone: 4G7). Cells were not fixed.
AFFINITY Figures
Affinity and Kinetic Characterization of 98088-1-RR
Biolayer interferometry (BLl) kinetic assays of 98088-1-RR against Human CD23 were performed. The affinity constant is below 1 pM.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
CD23 Recombinant antibody
Catalog Number
98088-1-RR
Citations
-
Dilutions
Applications
FC
Reactivity
human
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
