Anti-Human CD23 Rabbit Recombinant Antibody

CD23 Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

241270C6

Cat no : 98088-1-RR

Synonyms

C-type lectin domain family 4 member J, CLEC4J, CD23A, BLAST-2, BLAST 2

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Validation Data Gallery

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  FC experiment of human PBMCs using 98088-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD23 Rabbit Recombinant Antibody (98088-1-RR, Clone:241270C6) or Isotype Control and PE-conjugated Goat Anti-Rabbit IgG. Cell were then stained with CoraLite® Plus 647 Anti-Human CD19 (4G7). Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using 98088-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD23 Rabbit Recombinant Antibody (98088-1-RR, Clone:241270C6) (red) or Isotype Control (blue) and PE-conjugated Goat Anti-Rabbit IgG. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using 98088-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD23 Rabbit Recombinant Antibody (98088-1-RR, Clone:241270C6) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), and 0.25 ug Isotype Control, and 0.25 ug CoraLite® Plus 647 Anti-Human CD19 (4G7) Mouse IgG2a Recombinant Antibody (CL647-65562, Clone: 4G7). Cells were not fixed.

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  Affinity and Kinetic Characterization of 98088-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98088-1-RR against Human CD23 were performed. The affinity constant is below 1 pM.

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98088-1-RR targets CD23 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant Protein
• Full Name: Fc fragment of IgE, low affinity II, receptor for (CD23)
• Calculated Molecular Weight: 321 aa, 36 kDa
• GenBank Accession Number: BC064417
• Gene Symbol: CD23
• Gene ID (NCBI): 2208
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

CD23, also known as low affinity immunoglobulin epsilon Fc receptor, is a transmembrane glycoprotein present on a subpopulation of B lymphocytes in germinal centres, EBV-transformed B-lymphoblastoid cell lines, follicular dendritic cells, and a subpopulation of peripheral blood cells. CD23 has essential roles in the regulation of IgE production and in the differentiation of B-cells.