Anti-Human CD23 Rabbit Recombinant Antibody, PBS Only

CD23 Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

241270C6

Cat no : 98088-1-PBS

Synonyms

C-type lectin domain family 4 member J, CLEC4J, CD23A, BLAST-2, BLAST 2

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Validation Data Gallery

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  FC experiment of human PBMCs using 98088-1-RR (same clone as 98088-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD23 Rabbit Recombinant Antibody (98088-1-RR, Clone:241270C6) or Isotype Control and PE-conjugated Goat Anti-Rabbit IgG. Cell were then stained with CoraLite® Plus 647 Anti-Human CD19 (4G7). Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 98088-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 98088-1-RR (same clone as 98088-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD23 Rabbit Recombinant Antibody (98088-1-RR, Clone:241270C6) (red) or Isotype Control (blue) and PE-conjugated Goat Anti-Rabbit IgG. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 98088-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 98088-1-RR (same clone as 98088-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD23 Rabbit Recombinant Antibody (98088-1-RR, Clone:241270C6) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), and 0.25 ug Isotype Control, and 0.25 ug CoraLite® Plus 647 Anti-Human CD19 (4G7) Mouse IgG2a Recombinant Antibody (CL647-65562, Clone: 4G7). Cells were not fixed. This data was developed using the same antibody clone with 98088-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 98088-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98088-1-RR against Human CD23 were performed. The affinity constant is below 1 pM.

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Product Information

98088-1-PBS targets CD23 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant Protein
• Full Name: Fc fragment of IgE, low affinity II, receptor for (CD23)
• Calculated Molecular Weight: 321 aa, 36 kDa
• GenBank Accession Number: BC064417
• Gene Symbol: CD23
• Gene ID (NCBI): 2208
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CD23, also known as low affinity immunoglobulin epsilon Fc receptor, is a transmembrane glycoprotein present on a subpopulation of B lymphocytes in germinal centres, EBV-transformed B-lymphoblastoid cell lines, follicular dendritic cells, and a subpopulation of peripheral blood cells. CD23 has essential roles in the regulation of IgE production and in the differentiation of B-cells.