CoraLite® Plus 647 Anti-Mouse CD206 Rabbit Recombinant Antibody

CD206 Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

240344D12

Cat no : CL647-98031

Synonyms

Mrc1, MR, MMR, mannose receptor, C type 1, 240344D12

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Validation Data Gallery

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  FC experiment of mouse peritoneal macrophages using CL647-98031

  1x10^6 mouse peritoneal macrophages were surface stained with CoraLite® Plus 488 Anti-Mouse CD107b / LAMP2 and 0.25 ug CoraLite® Plus 647 Anti-Mouse CD206 Rabbit Recombinant Antibody (CL647-98031, Clone: 240344D12) or Isotype Control. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of mouse peritoneal macrophages using CL647-98031

  1x10^6 mouse peritoneal macrophages were surface stained with 0.25 ug CoraLite® Plus 647 Anti-Mouse CD206 Rabbit Recombinant Antibody (CL647-98031, Clone:240344D12) (red) or Isotype Control (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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Tested Applications

• Positive FC detected in: mouse peritoneal macrophages

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL647-98031 is CD206 Fusion Protein expressed in E. coli.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: mannose receptor, C type 1
• Calculated Molecular Weight: 165 kDa
• GenBank Accession Number: NM_008625.2
• Gene Symbol: CD206
• Gene ID (NCBI): 17533
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light.

Background Information

CD206, also named as MMR, CLEC13D and MRC1, is a type I membrane receptor that mediates the endocytosis of glycoproteins by macrophages. CD206 has been shown to bind high-mannose structures on the surface of potentially pathogenic viruses, bacteria, and fungi so that they can be neutralized by phagocytic engulfment. CD206 is a 170 kDa transmembrane protein which contains 5 domains: an amino-terminal cysteine-rich region, a fibronectin type II repeat, a series of eight tandem lectin-like carbohydrate recognition domains (responsible for the recognition of mannose and fucose), a transmembrane domain, and an intracellular carboxy-terminal tail. It is expressed on most tissue macrophages, in vitro derived dendritic cells, lymphatic and sinusoidal endothelia.