Anti-Human CD2 Rabbit Recombinant Antibody, PBS Only

CD2 Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

241222E2

Cat no : 98134-1-PBS

Synonyms

LFA-2, LFA 3 receptor, LFA 2, Erythrocyte receptor, CD2 molecule

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Validation Data Gallery

• 

  FC experiment of human PBMCs using 98134-1-RR (same clone as 98134-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD2 Rabbit Recombinant Antibody (98134-1-RR, Clone: 241222E2) or Isotype Control, and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody (APC-65569, Clone: OKT3). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98134-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 98134-1-RR (same clone as 98134-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD2 Rabbit Recombinant Antibody (98134-1-RR, Clone: 241222E2) (red) or Isotype Control (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98134-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 98134-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98134-1-RR against Human CD2 were performed. The affinity constant is 0.418 nM.

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Product Information

The immunogen of 98134-1-PBS is CD2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: CD2 molecule
• Calculated Molecular Weight: 351 aa, 39 kDa
• GenBank Accession Number: BC033583
• Gene Symbol: CD2
• Gene ID (NCBI): 914
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CD2 is a cell surface glycoprotein present on a majority of thymocytes, all mature T cells and subset of NK cells but not on B lymphocytes. It is a pan T-cell marker. CD2 interacts with lymphocyte function-associated antigen (LFA-3/CD58) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.