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Anti-Human CD2 Rabbit Recombinant Antibody

CD2 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

241222E2

Cat no : 98134-1-RR

Synonyms

LFA-2, LFA 3 receptor, LFA 2, Erythrocyte receptor, CD2 molecule

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD2 Rabbit Recombinant Antibody (98134-1-RR)

    FC experiment of human PBMCs using 98134-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD2 Rabbit Recombinant Antibody (98134-1-RR, Clone: 241222E2) or Isotype Control, and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody (APC-65569, Clone: OKT3). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human CD2 Rabbit Recombinant Antibody (98134-1-RR)

    FC experiment of human PBMCs using 98134-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD2 Rabbit Recombinant Antibody (98134-1-RR, Clone: 241222E2) (red) or Isotype Control (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • Affinity and Kinetic Characterization of 98134-1-RR

    Affinity and Kinetic Characterization of 98134-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98134-1-RR against Human CD2 were performed. The affinity constant is 0.418 nM.

Tested Applications

Positive FC detected inhuman PBMCs

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 98134-1-RR is CD2 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein
Full Name CD2 molecule
Calculated Molecular Weight 351 aa, 39 kDa
GenBank Accession NumberBC033583
Gene Symbol CD2
Gene ID (NCBI) 914
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

Background Information

CD2 is a cell surface glycoprotein present on a majority of thymocytes, all mature T cells and subset of NK cells but not on B lymphocytes. It is a pan T-cell marker. CD2 interacts with lymphocyte function-associated antigen (LFA-3/CD58) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.

Protocols

Product Specific Protocols
FC protocol for CD2 antibody 98134-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols