CoraLite® Plus 594 Anti-Human CD14 (UCHM-1)

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  FC experiment of human PBMCs using CL594-65056

  1x10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 594 Anti-Human CD14 (CL594-65056, Clone:UCHM-1) or CoraLite®594 Mouse IgG2a Isotype Control (CL594-65208, Clone: C1.18.4). Cells were treated with FC Receptor Block prior to staining. Cells were not fixed. Monocytes were gated.

• 

  FC experiment of human PBMCs using CL594-65056

  1x10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 594 Anti-Human CD14 (CL594-65056, Clone:UCHM-1) or CoraLite®594 Mouse IgG2a Isotype Control (CL594-65208, Clone: C1.18.4). Cells were treated with FC Receptor Block prior to staining. Cells were not fixed. Monocytes were gated.

CD14 Monoclonal Antibody for FC

Cat No. CL594-65056

Clone No.UCHM-1

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Synonyms

UCHM-1, Myeloid cell-specific leucine-rich glycoprotein, Monocyte differentiation antigen CD14, urinary form, Monocyte differentiation antigen CD14, membrane-bound form, CD14 molecule

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is N/A per 10^6 cells in a 100 µl suspension or N/A per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-65056 targets CD14 in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Thymocytes and peripheral blood lymphocytes from a remission AML patient
• Full Name: CD14 molecule
• Calculated Molecular Weight: 375 aa, 40 kDa
• GenBank Accession Number: BC010507
• Gene Symbol: CD14
• Gene ID (NCBI): 929
• ENSEMBL Gene ID: ENSG00000170458
• RRID: AB_3673435
• Conjugate: CoraLite® Plus 594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 594 nm / 615 nm
• Form: Liquid
• Purification Method: Affinity purification
• UNIPROT ID: P08571
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD14 is a 50-55 kDa glycosylphosphatidylinositol-anchored glycoprotein preferentially expressed on monocytes and macrophages, and at lower levels on granulocytes (PMID: 3385210; 2462937; 7685797). CD14 can also exist as a soluble protein. CD14 acts as a co-receptor for bacterial liposaccharides (LPS) (PMID: 1698311). It plays a major role in the inflammatory response of monocytes to LPS.