FITC Plus Anti-Human CD14 (UCHM-1)

CD14 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

Human and More (1)

Applications

FC

Conjugate

FITC Plus Fluorescent Dye

Publications

1

CloneNo.

UCHM-1

Cat no : FITC-65056

Synonyms

CD14, CD14 molecule

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Validation Data Gallery

• 

  FC experiment of human PBMCs using FITC-65056

  1X10^6 human PBMCs were surface stained with 5 ul FITC Plus Anti-Human CD14 (FITC-65056, Clone: UCHM-1) or unstained. Cells were not fixed. Monocytes were gated.

• 

  FC experiment of human PBMCs using FITC-65056

  1X10^6 human PBMCs were surface stained with 5 ul FITC Plus Anti-Human CD14 (FITC-65056, Clone: UCHM-1) or unstained. Cells were not fixed. Monocytes were gated.

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Published Applications

• FC: See 1 publications below

Product Information

FITC-65056 targets CD14 in FC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Thymocytes and peripheral blood lymphocytes from a remission AML patient
• Full Name: CD14 molecule
• Calculated Molecular Weight: 375 aa, 40 kDa
• GenBank Accession Number: BC010507
• Gene Symbol: CD14
• Gene ID (NCBI): 929
• RRID: AB_2883746
• Conjugate: FITC Plus Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 495 nm / 524 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD14 is a 50-55 kDa glycosylphosphatidylinositol-anchored glycoprotein preferentially expressed on monocytes and macrophages, and at lower levels on granulocytes (PMID: 3385210; 2462937; 7685797). CD14 can also exist as a soluble protein. CD14 acts as a co-receptor for bacterial liposaccharides (LPS) (PMID: 1698311). It plays a major role in the inflammatory response of monocytes to LPS.

Publications

Species	Application	Title
human	FC	J Bone Miner Res; Single-cell RNA sequencing reveals that an imbalance in monocyte subsets rather than changes in gene expression patterns is a feature of postmenopausal osteoporosis; Authors - Lin Tao