Tested Applications
| Positive FC detected in | human peripheral blood monocytes |
Recommended dilution
| Application | Dilution |
|---|---|
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IF | See 1 publications below |
Product Information
CL647-65056 targets CD14 in IF, FC applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Thymocytes and peripheral blood lymphocytes from a remission AML patient Predict reactive species |
| Full Name | CD14 molecule |
| Calculated Molecular Weight | 375 aa, 40 kDa |
| GenBank Accession Number | BC010507 |
| Gene Symbol | CD14 |
| Gene ID (NCBI) | 929 |
| ENSEMBL Gene ID | ENSG00000170458 |
| RRID | AB_2920246 |
| Conjugate | CoraLite® Plus 647 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 654 nm / 674 nm |
| Form | Liquid |
| Purification Method | Affinity purification |
| UNIPROT ID | P08571 |
| Storage Buffer | PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
CD14 is a 50-55 kDa glycosylphosphatidylinositol-anchored glycoprotein preferentially expressed on monocytes and macrophages, and at lower levels on granulocytes (PMID: 3385210; 2462937; 7685797). CD14 can also exist as a soluble protein. CD14 acts as a co-receptor for bacterial liposaccharides (LPS) (PMID: 1698311). It plays a major role in the inflammatory response of monocytes to LPS.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 647 CD14 antibody CL647-65056 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Oncol Lett Regulation of the migration of colorectal cancer stem cells via the TLR4/MyD88 signaling pathway by the novel surface marker CD14 following LPS stimulation |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Cyril (Verified Customer) (11-28-2024) | We tested the product on the Attune with excitation at 637 nm. I attempted to record the data on the RL1 filter (670/14), but it is only visible on RL2 (720/30). Since the cytometer is checked before each use, it is possible that the fluorochrome emits a shifted spectrum
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