CoraLite® Plus 647 Anti-Human CD14 (UCHM-1)

CD14 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

Human and More (1)

Applications

IF, FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Publications

1

CloneNo.

UCHM-1

Cat no : CL647-65056

Synonyms

CD14, CD14 molecule

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Validation Data Gallery

• 

  FC experiment of human peripheral blood monocytes using CL647-65056

  100 ul human peripheral blood were surface stained with CoraLite® Plus 647-conjugated Mouse IgG2a isotype control or 5.00 ul/test CoraLite® Plus 647 Anti-Human CD14 (CL647-65056, Clone: UCHM-1) and then treated with red blood cell lysis buffer. Cells were not fixed.

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Tested Applications

• Positive FC detected in: human peripheral blood monocytes

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

CL647-65056 targets CD14 in IF, FC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Thymocytes and peripheral blood lymphocytes from a remission AML patient
• Full Name: CD14 molecule
• Calculated Molecular Weight: 375 aa, 40 kDa
• GenBank Accession Number: BC010507
• Gene Symbol: CD14
• Gene ID (NCBI): 929
• RRID: AB_2920246
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD14 is a 50-55 kDa glycosylphosphatidylinositol-anchored glycoprotein preferentially expressed on monocytes and macrophages, and at lower levels on granulocytes (PMID: 3385210; 2462937; 7685797). CD14 can also exist as a soluble protein. CD14 acts as a co-receptor for bacterial liposaccharides (LPS) (PMID: 1698311). It plays a major role in the inflammatory response of monocytes to LPS.

Publications

Species	Application	Title
human	IF	Oncol Lett; Regulation of the migration of colorectal cancer stem cells via the TLR4/MyD88 signaling pathway by the novel surface marker CD14 following LPS stimulation; Authors - Yufei Li