CoraLite®594-conjugated CD11c/Integrin alpha X Recombinant antibody

CD11c/Integrin alpha X Uni-rAb^TM Recombinant Antibody for IF-P

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF-P

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

5O17

Cat no : CL594-81853

Synonyms

CD11c, ITGAX, Integrin alpha-X, Integrin alpha X, CD11 antigen-like family member C

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Validation Data Gallery

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  IF Staining of human tonsillitis using CL594-81853

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using CoraLite®594 CD11c/Integrin alpha X antibody (CL594-81853, Clone: 5O17 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IF-P detected in: human tonsillitis tissue

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-81853 targets CD11c/Integrin alpha X in IF-P applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CD11c/Integrin alpha X fusion protein Ag11380
• Full Name: integrin, alpha X (complement component 3 receptor 4 subunit)
• Calculated Molecular Weight: 1169 aa, 129 kDa
• GenBank Accession Number: BC038237
• Gene Symbol: CD11c
• Gene ID (NCBI): 3687
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11c, also known as integrin αX, is a type I transmembrane glycoprotein present on a variety of cells, including monocytes/macrophages, granulocytes, a subset of B cells, NK cells and dendritic cells (PMID: 2897326; 1680915; 1694698; 17389580). As a result of its high level of expression on most dendritic cells, CD11c is typically considered to be a marker of conventional dendritic cells (PMID: 27119555). CD11c forms an α/β heterodimer with CD18 (integrin β2). CD11c/CD18 acts a receptor for fibrinogen and is important in monocyte adhesion and chemotaxis (PMID: 1671533).