CD11c/Integrin alpha X Recombinant antibody

CD11c/Integrin alpha X Uni-rAb^TM Recombinant Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

5O17

Cat no : 81853-1-RR

Synonyms

CD11c, ITGAX, Integrin alpha-X, Integrin alpha X, CD11 antigen-like family member C

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Validation Data Gallery

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  IHC staining of human tonsillitis using 81853-1-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 81853-1-RR (CD11c/Integrin alpha X antibody) at dilution of 1:1500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 81853-1-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 81853-1-RR (CD11c/Integrin alpha X antibody) at dilution of 1:1500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of THP-1 using 81853-1-RR

  Immunofluorescent analysis of (4% PFA) fixed PMA treated THP-1 cells using CD11c/Integrin alpha X antibody (81853-1-RR, Clone: 5O17 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 81853-1-RR

  Biolayer interferometry (BLl) kinetic assays of 81853-1-RR against Human CD11c/Integrin alpha X were performed. The affinity constant is below 1 pM.

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Tested Applications

• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: PMA treated THP-1 cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:800-1:3200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

81853-1-RR targets CD11c/Integrin alpha X in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CD11c/Integrin alpha X fusion protein Ag11380
• Full Name: integrin, alpha X (complement component 3 receptor 4 subunit)
• Calculated Molecular Weight: 1169 aa, 129 kDa
• GenBank Accession Number: BC038237
• Gene Symbol: CD11c
• Gene ID (NCBI): 3687
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11c, also known as integrin αX, is a type I transmembrane glycoprotein present on a variety of cells, including monocytes/macrophages, granulocytes, a subset of B cells, NK cells and dendritic cells (PMID: 2897326; 1680915; 1694698; 17389580). As a result of its high level of expression on most dendritic cells, CD11c is typically considered to be a marker of conventional dendritic cells (PMID: 27119555). CD11c forms an α/β heterodimer with CD18 (integrin β2). CD11c/CD18 acts a receptor for fibrinogen and is important in monocyte adhesion and chemotaxis (PMID: 1671533).

Publications

Species	Application	Title
rat	IF	FASEB J; Metformin switches cell death modes to soothe the apical periodontitis via ZBP1; Authors - Hui Liu