CD11c/Integrin alpha X Recombinant antibody, PBS Only

• 

  IHC staining of human tonsillitis using 81853-1-RR (same clone as 81853-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 81853-1-RR (CD11c/Integrin alpha X antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81853-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 81853-1-RR (same clone as 81853-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 81853-1-RR (CD11c/Integrin alpha X antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81853-1-PBS in a different storage buffer formulation.

• 

  IF Staining of THP-1 using 81853-1-RR (same clone as 81853-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed PMA treated THP-1 cells using CD11c/Integrin alpha X antibody (81853-1-RR, Clone: 5O17 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 81853-1-PBS in a different storage buffer formulation.

CD11c/Integrin alpha X Uni-rAb^TM Recombinant Antibody for IHC, IF/ICC, Indirect ELISA

Cat No. 81853-1-PBS

Clone No.5O17

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, Indirect ELISA

Synonyms

CD11c, ITGAX, Integrin alpha-X, Integrin alpha X, CD11 antigen-like family member C

---

Product Information

81853-1-PBS targets CD11c/Integrin alpha X in IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CD11c/Integrin alpha X fusion protein Ag11380
• Full Name: integrin, alpha X (complement component 3 receptor 4 subunit)
• Calculated Molecular Weight: 1169 aa, 129 kDa
• GenBank Accession Number: BC038237
• Gene Symbol: CD11c
• Gene ID (NCBI): 3687
• ENSEMBL Gene ID: ENSG00000140678
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P20702
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11c, also known as integrin αX, is a type I transmembrane glycoprotein present on a variety of cells, including monocytes/macrophages, granulocytes, a subset of B cells, NK cells and dendritic cells (PMID: 2897326; 1680915; 1694698; 17389580). As a result of its high level of expression on most dendritic cells, CD11c is typically considered to be a marker of conventional dendritic cells (PMID: 27119555). CD11c forms an α/β heterodimer with CD18 (integrin β2). CD11c/CD18 acts a receptor for fibrinogen and is important in monocyte adhesion and chemotaxis (PMID: 1671533).