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CD11c/Integrin alpha X Recombinant antibody, PBS Only

CD11c/Integrin alpha X Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5O17

Cat no : 81853-1-PBS

Synonyms

CD11c, ITGAX, Integrin alpha-X, Integrin alpha X, CD11 antigen-like family member C

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD11c/Integrin alpha X Recombinant antibody (81853-1-RR)

    IHC staining of human tonsillitis using 81853-1-RR (same clone as 81853-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 81853-1-RR (CD11c/Integrin alpha X antibody) at dilution of 1:1500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81853-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD11c/Integrin alpha X Recombinant antibody (81853-1-RR)

    IHC staining of human tonsillitis using 81853-1-RR (same clone as 81853-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 81853-1-RR (CD11c/Integrin alpha X antibody) at dilution of 1:1500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81853-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of THP-1 cells using CD11c/Integrin alpha X Recombinant antibody (81853-1-RR)

    IF Staining of THP-1 using 81853-1-RR (same clone as 81853-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed PMA treated THP-1 cells using CD11c/Integrin alpha X antibody (81853-1-RR, Clone: 5O17 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 81853-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 81853-1-RR

    Affinity and Kinetic Characterization of 81853-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81853-1-RR against Human CD11c/Integrin alpha X were performed. The affinity constant is below 1 pM.

Product Information

81853-1-PBS targets CD11c/Integrin alpha X in IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CD11c/Integrin alpha X fusion protein Ag11380
Full Name integrin, alpha X (complement component 3 receptor 4 subunit)
Calculated Molecular Weight 1169 aa, 129 kDa
GenBank Accession NumberBC038237
Gene Symbol CD11c
Gene ID (NCBI) 3687
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11c, also known as integrin αX, is a type I transmembrane glycoprotein present on a variety of cells, including monocytes/macrophages, granulocytes, a subset of B cells, NK cells and dendritic cells (PMID: 2897326; 1680915; 1694698; 17389580). As a result of its high level of expression on most dendritic cells, CD11c is typically considered to be a marker of conventional dendritic cells (PMID: 27119555). CD11c forms an α/β heterodimer with CD18 (integrin β2). CD11c/CD18 acts a receptor for fibrinogen and is important in monocyte adhesion and chemotaxis (PMID: 1671533).