CoraLite® Plus 488 Anti-Mouse CD11c (N418)

CD11c Monoclonal Antibody for IF/ICC, FC

Host / Isotype

Armenian Hamster / IgG

Reactivity

Mouse

Applications

IF/ICC, FC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

N418

Cat no : CL488-65130

Synonyms

AI449405, Cd11c, Cr4, integrin alpha X, Itgax, N418

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Validation Data Gallery

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  IF Staining of mouse splenocytes using CL488-65130

  Immunofluorescent analysis of mouse splenocytes using CoraLite® Plus 488 CD11c antibody (CL488-65130, Clone: N418 ) at dilution of 1:400.

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  FC experiment of mouse splenocytes using CL488-65130

  1X10^6 mouse splenocytes were surface co-stained with APC Anti-Mouse CD45R (B220) (RA3-6B2) and 0.25 ug CoraLite® Plus 488 Anti-Mouse CD11c (CL488-65130, Clone: N418) or 0.25 ug isotype control. Cells were not fixed.

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Tested Applications

• Positive IF/ICC detected in: mouse splenocytes
• Positive FC detected in: mouse splenocytes

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-65130 targets CD11c in IF/ICC, FC applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Host / Isotype: Armenian Hamster / IgG
• Class: Monoclonal
• Type: Antibody
• Immunogen: Mouse spleen dendritic cells
• Full Name: integrin alpha X
• GenBank Accession Number: BC167225
• Gene Symbol: CD11c
• Gene ID (NCBI): 16411
• RRID: AB_2883193
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11c, also known as integrin αX, is a type I transmembrane glycoprotein present on a variety of cells, including monocytes/macrophages, granulocytes, a subset of B cells, NK cells and dendritic cells (PMID: 2897326; 1680915; 1694698; 17389580). As a result of its high level of expression on most dendritic cells, CD11c is typically considered to be a marker of conventional dendritic cells (PMID: 27119555). CD11c forms an α/β heterodimer with CD18 (integrin β2). CD11c/CD18 acts a receptor for fibrinogen and is important in monocyte adhesion and chemotaxis (PMID: 1671533).