APC Anti-Mouse CD11c (N418)

CD11c Monoclonal Antibody for FC

Host / Isotype

Armenian Hamster / IgG

Reactivity

Mouse and More (1)

Applications

FC

Conjugate

APC Fluorescent Dye

Publications

4

CloneNo.

N418

Cat no : APC-65130

Synonyms

AI449405, Cd11c, Cr4, integrin alpha X, Itgax, N418

---

Validation Data Gallery

• 

  FC experiment of mouse splenocytes using APC-65130

  1X10^6 mouse splenocytes were surface co-stained with FITC Anti-Mouse MHC Class II (I-A/I-E) and 0.2 ug APC Anti-Mouse CD11c (APC-65130, Clone: N418) or 0.2 ug Isotype Control. Cells were not fixed.

---

Tested Applications

• Positive FC detected in: mouse splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

• FC: See 3 publications below

Product Information

APC-65130 targets CD11c in FC applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Cited Reactivity: mouse
• Host / Isotype: Armenian Hamster / IgG
• Class: Monoclonal
• Type: Antibody
• Immunogen: Mouse spleen dendritic cells
• Full Name: integrin alpha X
• GenBank Accession Number: BC167225
• Gene Symbol: CD11c
• Gene ID (NCBI): 16411
• RRID: AB_2883012
• Conjugate: APC Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 650 nm / 660 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.1% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11c, also known as integrin αX, is a type I transmembrane glycoprotein present on a variety of cells, including monocytes/macrophages, granulocytes, a subset of B cells, NK cells and dendritic cells (PMID: 2897326; 1680915; 1694698; 17389580). As a result of its high level of expression on most dendritic cells, CD11c is typically considered to be a marker of conventional dendritic cells (PMID: 27119555). CD11c forms an α/β heterodimer with CD18 (integrin β2). CD11c/CD18 acts a receptor for fibrinogen and is important in monocyte adhesion and chemotaxis (PMID: 1671533).

Publications

Species	Application	Title
		J Control Release; Programmed antigen capture-harnessed dendritic cells by margination-hitchhiking lung delivery; Authors - Thi My Hue Huynh
mouse	FC	Drug Deliv; Sonodynamical reversion of immunosuppressive microenvironment in prostate cancer via engineered exosomes.; Authors - Dingyi Wang
mouse	FC	Pharmaceutics; Development and Evaluation of a Novel Diammonium Glycyrrhizinate Phytosome for Nasal Vaccination; Authors - Xiaojin Chen
mouse	FC	Asian J Pharm Sci; Lignin-assisted construction of sub-10 nm supramolecular self-assembly for photothermal immunotherapy and potentiating anti-PD-1 therapy against primary and distant breast tumors; Authors - Xudong Fan