PE Anti-Mouse CD11c (N418)

CD11c Monoclonal Antibody for FC

Host / Isotype

Armenian Hamster / IgG

Reactivity

Mouse and More (1)

Applications

FC

Conjugate

PE Fluorescent Dye

Publications

3

CloneNo.

N418

Cat no : PE-65130

Synonyms

AI449405, Cd11c, Cr4, integrin alpha X, Itgax, N418

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Validation Data Gallery

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  FC experiment of mouse splenocytes using PE-65130

  1X10^6 mouse splenocytes were surface co-stained with APC Anti-Mouse MHC Class II (I-A/I-E) (M5/114.15.2) and 0.25 ug PE Anti-Mouse CD11c (PE-65130, Clone: N418) or 0.25 ug PE Armenian Hamster IgG Isotype Control. Cells were not fixed.

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Tested Applications

• Positive FC detected in: mouse splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

• FC: See 3 publications below

Product Information

PE-65130 targets CD11c in FC applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Cited Reactivity: mouse
• Host / Isotype: Armenian Hamster / IgG
• Class: Monoclonal
• Type: Antibody
• Immunogen: Mouse spleen dendritic cells
• Full Name: integrin alpha X
• GenBank Accession Number: BC167225
• Gene Symbol: CD11c
• Gene ID (NCBI): 16411
• RRID: AB_2883914
• Conjugate: PE Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.1% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11c, also known as integrin αX, is a type I transmembrane glycoprotein present on a variety of cells, including monocytes/macrophages, granulocytes, a subset of B cells, NK cells and dendritic cells (PMID: 2897326; 1680915; 1694698; 17389580). As a result of its high level of expression on most dendritic cells, CD11c is typically considered to be a marker of conventional dendritic cells (PMID: 27119555). CD11c forms an α/β heterodimer with CD18 (integrin β2). CD11c/CD18 acts a receptor for fibrinogen and is important in monocyte adhesion and chemotaxis (PMID: 1671533).

Publications

Species	Application	Title
mouse	FC	Nutrients; The Potential Role of Moringa oleifera Lam. Leaf Proteins in Moringa Allergy by Functionally Activating Murine Bone Marrow-Derived Dendritic Cells and Inducing Their Differentiation toward a Th2-Polarizing Phenotype; Authors - Chuyu Xi
mouse	FC	Adv Healthc Mater; Pathogen-mimicking Nanoparticles Based on Rigid Nanomaterials as an Efficient Subunit Vaccine Delivery System for Intranasal Iimmunization; Authors - Hongping Wan
mouse	FC	Int J Biol Macromol; Mucoadhesive chitosan-catechol as an efficient vaccine delivery system for intranasal immunization; Authors - Kai Deng