CoraLite® Plus 488 Anti-Human CD11b (ICRF44)

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  IF Staining of human peripheral blood mononuclear cells using CL488-65116

  Immunofluorescent analysis of human peripheral blood mononuclear cells (PBMCs) using CoraLite® Plus 488-conjugated Anti-Human CD11b (CL488-65116, Clone: ICRF44) at dilution of 1:400.

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  FC experiment of human PBMCs using CL488-65116

  1x10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 488 Anti-Human CD11b (ICRF44) (CL488-65116, Clone: ICRF44) or CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) . Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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  FC experiment of human PBMCs using CL488-65116

  1x10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 488 Anti-Human CD11b (ICRF44) (CL488-65116, Clone: ICRF44) (red), or CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

CD11b Monoclonal Antibody for IF/ICC, FC

Cat No. CL488-65116

Clone No.ICRF44

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human

Applications

IF/ICC, FC

Synonyms

ICRF44, CR3A, CR-3 alpha chain, CR 3 alpha chain, Cell surface glycoprotein MAC-1 subunit alpha

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Tested Applications

• Positive IF/ICC detected in: human peripheral blood mononuclear cells
• Positive FC detected in: human PBMCs

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC): FC : 5 ul per 10^6 cells in 100 μl suspension

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-65116 targets CD11b in IF/ICC, FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Rheumatoid synovial cells and human monocytes
• Full Name: integrin, alpha M (complement component 3 receptor 3 subunit)
• Calculated Molecular Weight: 1152 aa, 127 kDa
• GenBank Accession Number: BC096346
• Gene Symbol: CD11b
• Gene ID (NCBI): 3684
• ENSEMBL Gene ID: ENSG00000169896
• RRID: AB_2883188
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Affinity purification
• UNIPROT ID: P11215
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11b, also known as Integrin alpha M or CR3A, belongs to the integrin alpha chain family. CD11b forms an α/β heterodimer with CD18 (integrin β2). CD11b/CD18 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PMID: 9558116; 20008295). CD11b/CD18 is a receptor for the complement protein fragment iC3b, and is also a receptor for fibrinogen, factor X and ICAM1 (PMID: 2971974; 15485828).