Tested Applications
| Positive FC detected in | human PBMCs |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 5 ul per 10^6 cells in 100 μl suspension |
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
FITC-65582 targets CD11b in FC applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG2a |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Rheumatoid synovial cells and human monocytes Predict reactive species |
| Full Name | integrin, alpha M (complement component 3 receptor 3 subunit) |
| Calculated Molecular Weight | 1152 aa, 127 kDa |
| GenBank Accession Number | BC096346 |
| Gene Symbol | CD11b |
| Gene ID (NCBI) | 3684 |
| ENSEMBL Gene ID | ENSG00000169896 |
| RRID | AB_3749270 |
| Conjugate | FITC Plus Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 495 nm / 524 nm |
| Excitation Laser | Blue laser (488 nm) |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P11215 |
| Storage Buffer | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits (PMID: 9779984). CD11b, also known as Integrin alpha M or CR3A, belongs to the integrin alpha chain family. CD11b forms an α/β heterodimer with CD18 (integrin β2). CD11b/CD18 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (PMID: 9558116; 20008295). CD11b/CD18 is a receptor for the complement protein fragment iC3b, and is also a receptor for fibrinogen, factor X and ICAM1 (PMID: 2971974; 15485828).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for FITC Plus CD11b antibody FITC-65582 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
FITC-65582 is the Anti-Human CD11b (ICRF44) Mouse IgG2a Recombinant Antibody. It has a total of 1 citation, published in Biomedicines (IF 4.5). The cited study investigated targeting the glutamine transporter SLC1A5 to enhance sensitivity of acute myeloid leukemia cells to MLN4924, using flow cytometry on human samples. The research field encompasses leukemia biology, integrin-mediated cell adhesion, and immunotherapy.
| Species | Application | Title |
|---|---|---|
Biomedicines Targeting the Glutamine Transporter SLC1A5 Enhances Sensitivity of Acute Myeloid Leukemia to MLN4924. |
