CoraLite® Plus 647 Anti-Human CD107b / LAMP2 (H4B4) Rabbit IgG Recombinant Antibody

CD107b / LAMP2 Recombinant Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

H4B4

Cat no : CL647-65520

Synonyms

CD107b, LAMP 2, LAMP2, LAMPB, LGP110

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Validation Data Gallery

• 

  FC experiment of human PBMCs using CL647-65520

  1x10^6 human PBMCs were intracellularly stained with 5 ul CoraLite® Plus 647 Anti-Human CD107b Rabbit Recombinant Antibody (CL647-65520, Clone:H4B4) or CoraLite® Plus 647-conjugated Rabbit IgG control PolyAb (CL647-30000). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Cells were treated with FC Receptor Block prior to staining.

• 

  FC experiment of human PBMCs using CL647-65520

  1x10^6 human PBMCs were intracellularly stained with 5 ul CoraLite® Plus 647 Anti-Human CD107b Rabbit Recombinant Antibody (CL647-65520, Clone:H4B4) or CoraLite® Plus 647-conjugated Rabbit IgG control PolyAb (CL647-30000). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Cells were treated with FC Receptor Block prior to staining.

• 

  FC experiment of human PBMCs using CL647-65520

  1x10^6 human PBMCs were intracellularly stained with 5 ul CoraLite® Plus 647 Anti-Human CD107b Rabbit Recombinant Antibody (CL647-65520, Clone:H4B4) or 5 ul CoraLite® Plus 647 Anti-Human CD107b Mouse Monoclonal Antibody (CL647-65053, Clone:H4B4). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). Cells were treated with FC Receptor Block prior to staining.

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

Application	Dilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-65520 targets CD107b / LAMP2 in FC (Intra) applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Human adherent peripheral blood cells
• Full Name: lysosomal-associated membrane protein 2
• Calculated Molecular Weight: 45 kDa
• GenBank Accession Number: BC002965
• Gene Symbol: LAMP2
• Gene ID (NCBI): 3920
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

LAMP2 (CD107b) is a Lysosomal membrane glycoprotein. LAMP2 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP-2 is localized primarily in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane under certain circumstances, e.g., after platelet activation, during granulocytic differentiation and activation, and in some tumor cells (PMID: 12221139). LAMP is involved in lysosomal stability and autophagy (PMID: 12221139). This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may play a role in tumor cell metastasis (PMID 9426697).