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CoraLite® Plus 647 Anti-Human CD107b / LAMP2 (H4B4)

CD107b / LAMP2 Monoclonal Antibody for IF, FC (Intra)

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

H4B4

Cat no : CL647-65053

Synonyms

CD107b, Cell and organelle markers, LAMP 2, LAMP2, LAMPB, LGP110, Lysosome Marker

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 647 Anti-Human CD107b / LAMP2 (H4B4 (CL647-65053)

    IF Staining of HeLa using CL647-65053

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 647-conjugated CD107b antibody (CL647-65053, Clone: H4B4) at dilution of 1:50 and CoraLite®488-conjugated CD107a antibody (CL488-65051, Clone: H4A3, green) at dilution of 1:50.

  • Flow cytometry (FC) experiment of Jurkat cells using CoraLite® Plus 647 Anti-Human CD107b / LAMP2 (H4B4 (CL647-65053)

    FC experiment of Jurkat using CL647-65053

    1X10^6 Jurkat cells were intracellularly stained with 5 ul CoraLite® Plus 647 Anti-Human CD107b (CL647-65053, Clone:H4B4) (red) or Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF detected inHeLa cells
Positive FC detected inJurkat cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-65053 targets CD107b / LAMP2 in IF, FC (Intra) applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen Human adherent peripheral blood cells
Full Name lysosomal-associated membrane protein 2
Calculated Molecular Weight 45 kDa
GenBank Accession NumberBC002965
Gene Symbol LAMP2
Gene ID (NCBI) 3920
RRIDAB_2883656
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

LAMP2 (CD107b) is a Lysosomal membrane glycoprotein. LAMP2 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP-2 is localized primarily in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane under certain circumstances, e.g., after platelet activation, during granulocytic differentiation and activation, and in some tumor cells (PMID: 12221139). LAMP is involved in lysosomal stability and autophagy (PMID: 12221139). This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may play a role in tumor cell metastasis (PMID 9426697).

Protocols

Product Specific Protocols
IF protocol for CL Plus 647 CD107b / LAMP2 antibody CL647-65053Download protocol
Standard Protocols
Click here to view our Standard Protocols