Anti-Mouse CD107a / LAMP1 (1D4B), PBS Only

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  IF Staining of NIH/3T3 using 65050-1-Ig (same clone as 65050-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using 65050-1-Ig (CD107a antibody) at dilution of 1:100 and Rhodamine (TRITC)-conjugated Goat Anti-Rat IgG(H+L). This data was developed using the same antibody clone with 65050-1-PBS in a different storage buffer formulation.

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  FC experiment of NIH/3T3 using 65050-1-Ig (same clone as 65050-1-PBS)

  1X10^6 NIH/3T3 cells were intracellularly stained with 0.5 ug Anti-Mouse CD107a / LAMP1 (65050-1-Ig, Clone:1D4B) and FITC anti-Rat IgG2a antibody at dilution 1:100, or 0.5 ug Rat IgG2a Isotype Control (2A3) (65209-1-Ig, Clone: 2A3) and FITC anti-Rat IgG2a antibody at dilution 1:100. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 65050-1-PBS in a different storage buffer formulation.

CD107a / LAMP1 Monoclonal Antibody for IF/ICC, FC (Intra)

Cat No. 65050-1-PBS

Clone No.1D4B

Host / Isotype

Rat / IgG2a, kappa

Reactivity

mouse

Applications

IF/ICC, FC (Intra)

Synonyms

CD107a, Lamp1, Lamp 1, 1D4B

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Product Information

65050-1-PBS targets CD107a / LAMP1 in IF/ICC, FC (Intra) applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rat / IgG2a, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Plasma membrane fraction of mouse embryo NIH/3T3 cell line
• Full Name: lysosomal-associated membrane protein 1
• GenBank Accession Number: BC006785
• Gene Symbol: CD107a
• Gene ID (NCBI): 16783
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P11438
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

LAMP1 (CD107a) is a heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP1 is expressed largely in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane (PMID: 16168398). Elevated LAMP1 expression at the cell surface has also been detected during platelet and granulocytic cell activation, as well as in some tumor cells (PMID: 29085473). LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis (PMID: 18835598; 29085473; 9426697).