Anti-Mouse CD107a / LAMP1 (1D4B)

CD107a / LAMP1 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rat / IgG2a, kappa

Reactivity

Mouse and More (1)

Applications

IF/ICC, FC (Intra)

Conjugate

Unconjugated

Publications

4

CloneNo.

1D4B

Cat no : 65050-1-Ig

Synonyms

AI196048, CD107a, Lamp 1, Lamp1

---

Validation Data Gallery

• 

  IF Staining of NIH/3T3 using 65050-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using 65050-1-Ig (CD107a antibody) at dilution of 1:100 and Rhodamine (TRITC)-conjugated Goat Anti-Rat IgG(H+L).

• 

  FC experiment of NIH/3T3 using 65050-1-Ig

  1X10^6 NIH/3T3 cells were intracellularly stained with 0.5 ug Anti-Mouse CD107a / LAMP1 (65050-1-Ig, Clone:1D4B) and FITC anti-Rat IgG2a antibody at dilution 1:100, or 0.5 ug Rat IgG2a Isotype Control (2A3) (65209-1-Ig, Clone: 2A3) and FITC anti-Rat IgG2a antibody at dilution 1:100. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Tested Applications

• Positive IF/ICC detected in: NIH/3T3 cells
• Positive FC (Intra) detected in: NIH/3T3 cells
• Positive FC detected in: NIH/3T3 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 4 publications below

Product Information

65050-1-Ig targets CD107a / LAMP1 in IF/ICC, FC (Intra) applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Cited Reactivity: mouse
• Host / Isotype: Rat / IgG2a, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Plasma membrane fraction of mouse embryo NIH/3T3 cell line
• Full Name: lysosomal-associated membrane protein 1
• GenBank Accession Number: BC006785
• Gene Symbol: CD107a
• Gene ID (NCBI): 16783
• RRID: AB_2881466
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

LAMP1 (CD107a) is a heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP1 is expressed largely in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane (PMID: 16168398). Elevated LAMP1 expression at the cell surface has also been detected during platelet and granulocytic cell activation, as well as in some tumor cells (PMID: 29085473). LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis (PMID: 18835598; 29085473; 9426697).

Publications

Species	Application	Title
mouse	IF	J Cell Physiol; Identification of circular dorsal ruffles as signal platforms for the AKT pathway in glomerular podocytes; Authors - Rui Hua
mouse	IF	Res Sq; Pathological characteristics of axons and proteome patterns in midbrain dopaminergic neurodegeneration induced by WDR45-deficiency; Authors - Weidong Le
mouse	IF	J Leukoc Biol; Macropinocytic cups function as signal platforms for the mTORC2-AKT pathway to modulate LPS-induced cytokine expression in macrophages; Authors - Li Wang
mouse	IF	Mol Neurodegener; Pathological characteristics of axons and alterations of proteomic and lipidomic profiles in midbrain dopaminergic neurodegeneration induced by WDR45-deficiency; Authors - Panpan Wang