Anti-Mouse CD107a / LAMP1 (1D4B)

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  IF Staining of NIH/3T3 using 65050-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using 65050-1-Ig (CD107a antibody) at dilution of 1:100 and Rhodamine (TRITC)-conjugated Goat Anti-Rat IgG(H+L).

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  FC experiment of NIH/3T3 using 65050-1-Ig

  1X10^6 NIH/3T3 cells were intracellularly stained with 0.5 ug Anti-Mouse CD107a / LAMP1 (65050-1-Ig, Clone:1D4B) and FITC anti-Rat IgG2a antibody at dilution 1:100, or 0.5 ug Rat IgG2a Isotype Control (2A3) (65209-1-Ig, Clone: 2A3) and FITC anti-Rat IgG2a antibody at dilution 1:100. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CD107a / LAMP1 Monoclonal Antibody for IF/ICC, FC (Intra)

Cat No. 65050-1-Ig

Clone No.1D4B

Publications(4)

Host / Isotype

Rat / IgG2a, kappa

Reactivity

mouse

Applications

IF/ICC, FC (Intra)

Synonyms

CD107a, Lamp1, Lamp 1, 1D4B

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Tested Applications

• Positive IF/ICC detected in: NIH/3T3 cells
• Positive FC (Intra) detected in: NIH/3T3 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.5 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 4 publications below

Product Information

65050-1-Ig targets CD107a / LAMP1 in IF/ICC, FC (Intra) applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Cited Reactivity: mouse
• Host / Isotype: Rat / IgG2a, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Plasma membrane fraction of mouse embryo NIH/3T3 cell line
• Full Name: lysosomal-associated membrane protein 1
• GenBank Accession Number: BC006785
• Gene Symbol: CD107a
• Gene ID (NCBI): 16783
• RRID: AB_2881466
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P11438
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

LAMP1 (CD107a) is a heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 is extensively glycosylated with asparagine-linked oligosaccharides which protect it from intracellular proteolysis (PMID: 10521503). Although LAMP1 is expressed largely in the endosome-lysosomal membrane of cells, it is also found on the plasma membrane (PMID: 16168398). Elevated LAMP1 expression at the cell surface has also been detected during platelet and granulocytic cell activation, as well as in some tumor cells (PMID: 29085473). LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis (PMID: 18835598; 29085473; 9426697).

Publications

Species	Application	Title
mouse	IF	J Cell Physiol; Identification of circular dorsal ruffles as signal platforms for the AKT pathway in glomerular podocytes; Authors - Rui Hua
mouse	IF	Res Sq; Pathological characteristics of axons and proteome patterns in midbrain dopaminergic neurodegeneration induced by WDR45-deficiency; Authors - Weidong Le
mouse	IF	J Leukoc Biol; Macropinocytic cups function as signal platforms for the mTORC2-AKT pathway to modulate LPS-induced cytokine expression in macrophages; Authors - Li Wang
mouse	IF	Mol Neurodegener; Pathological characteristics of axons and alterations of proteomic and lipidomic profiles in midbrain dopaminergic neurodegeneration induced by WDR45-deficiency; Authors - Panpan Wang