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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Rat IgG2a Isotype Control (2A3)

IgG2a Isotype Control Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2a, kappa

Reactivity

n/a

Applications

FC

Conjugate

Unconjugated

CloneNo.

2A3

Cat no : 65209-1-Ig

Synonyms

Rat IgG2a Isotype Control, IgG2a

Validation Data Gallery

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  • Flow cytometry (FC) experiment of mouse splenocytes using Rat IgG2a Isotype Control (2A3) (65209-1-Ig)

    FC experiment of mouse splenocytes using 65209-1-Ig

    1x10^6 mouse splenocytes were surface stained with 0.25 ug Rat IgG2a Isotype Control (2A3) (65209-1-Ig, Clone: 2A3) and FITC anti-rat IgG2a Antibody (red), or 0.25 ug Anti-Mouse CD8a (65069-1-Ig, Clone: 53-6.7) and FITC anti-rat IgG2a Antibody (green) or unstained (blue). Cells were not fixed.

Tested Applications

Positive FC detected in

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65209-1-Ig targets IgG2a Isotype Control in FC applications and shows reactivity with n/a samples.

Tested Reactivity n/a
Host / Isotype Rat / IgG2a, kappa
Class Monoclonal
Type Antibody
Immunogen N/A
Full Name IgG2a Isotype Control
Gene Symbol
Gene ID (NCBI)
RRIDAB_2918471
Conjugate Unconjugated
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

The 2A3 immunoglobulin is useful as an isotype-matched control. The 2A3 immunolglobulin binds to TNP (trinitrophenol), a hapten not expressed on mouse or human cells. It is used as an isotype control for rat IgG2a antibodies. This antibody has been quality-tested for flow cytometry as negative control.

Protocols

Product Specific Protocols
FC protocol for IgG2a Isotype Control antibody 65209-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols