CoraLite® Plus 488-conjugated CD107a / LAMP1 Monoclonal antibody

CD107a / LAMP1 Monoclonal Antibody for FC (Intra), IF

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3B2E9

Cat no : CL488-67300

Synonyms

CD107a, Cell and organelle markers, LAMP 1, LAMP1, LAMPA, LGP120, lysosomal marker, Lysosome Marker



Tested Applications

Positive IF detected inhuman liver cancer tissue
Positive FC detected inNK92

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67300 targets CD107a / LAMP1 in IF, FC (Intra) applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CD107a / LAMP1 fusion protein Ag28869
Full Name lysosomal-associated membrane protein 1
Calculated Molecular Weight 417 aa, 45 kDa
Observed Molecular Weight 90-120 kDa
GenBank Accession NumberBC021288
Gene Symbol LAMP1
Gene ID (NCBI) 3916
RRIDAB_2919446
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LAMP1 (also known as CD107a) is a 90-120 kDa heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 CD107a / LAMP1 antibody CL488-67300Download protocol
FC protocol for CL Plus 488 CD107a / LAMP1 antibody CL488-67300Download protocol
Standard Protocols
Click here to view our Standard Protocols