CoraLite®594-conjugated CD107a / LAMP1 Monoclonal antibody

CD107a / LAMP1 Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG2b

Reactivity

human and More (1)

Applications

IF-P

Conjugate

CoraLite®594 Fluorescent Dye

Publications

2

CloneNo.

3B2E9

Cat no : CL594-67300

Synonyms

CD107a, LAMP1, LAMP-1, LAMP 1, CD107 antigen-like family member A

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Validation Data Gallery

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  IF Staining of human liver cancer using CL594-67300

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite®594 CD107a antibody (CL594-67300, Clone: 3B2E9 ) at dilution of 1:200.

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  IF Staining of human liver cancer using CL594-67300

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite®594 CD107a antibody (CL594-67300, Clone: 3B2E9 ) at dilution of 1:200.

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Tested Applications

• Positive IF-P detected in: human liver cancer tissue

This antibody is not recommended for immunocytofluorescent assays.

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 2 publications below

Product Information

CL594-67300 targets CD107a / LAMP1 in IF-P applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: CD107a / LAMP1 fusion protein Ag28869
• Full Name: lysosomal-associated membrane protein 1
• Calculated Molecular Weight: 417 aa, 45 kDa
• Observed Molecular Weight: 90-120 kDa
• GenBank Accession Number: BC021288
• Gene Symbol: LAMP1
• Gene ID (NCBI): 3916
• RRID: AB_2920108
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

LAMP1 (also known as CD107a) is a 90-120 kDa heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis.

Publications

Species	Application	Title
mouse	IF	J Adv Res; Ceramide-1-phosphate alleviates high-altitude pulmonary edema by stabilizing circadian ARNTL-mediated mitochondrial dynamics; Authors - Liuyang Tian
human,mouse	IF	Front Pharmacol; Low-dose of caffeine alleviates high altitude pulmonary edema via regulating mitochondrial quality control process in AT1 cells; Authors - Liuyang Tian