CBX1 Recombinant antibody

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  WB analysis using 83248-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83248-3-RR (CBX1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 83248-3-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CBX1 antibody (83248-3-RR, Clone: 240075E1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

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  IF Staining of HeLa using 83248-3-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CBX1 antibody (83248-3-RR, Clone: 240075E1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

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  IF Staining of A431 using 83248-3-RR

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CBX1 antibody (83248-3-RR, Clone: 240075E1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

• 

  IF Staining of A431 using 83248-3-RR

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CBX1 antibody (83248-3-RR, Clone: 240075E1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

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  FC experiment of HeLa using 83248-3-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug CBX1 Recombinant antibody (83248-3-RR, Clone:240075E1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  Affinity and Kinetic Characterization of 83248-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83248-3-RR against Human CBX1 were performed. The affinity constant is 3.31 nM.

CBX1 Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Cat No. 83248-3-RR

Clone No.240075E1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

HP1Hs beta, HP1 beta, Heterochromatin protein p25, Chromobox protein homolog 1, CBX

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Tested Applications

• Positive WB detected in: CHO cells, PC-12 cells, MCF-7 cells, NIH/3T3 cells, mouse spleen tissue, mouse brain tissue
• Positive IF/ICC detected in: HeLa cells, A431 cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83248-3-RR targets CBX1 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CBX1 fusion protein Ag34901
• Full Name: chromobox homolog 1 (HP1 beta homolog Drosophila )
• Calculated Molecular Weight: 27 kDa
• Observed Molecular Weight: 27 kDa
• GenBank Accession Number: BC002609
• Gene Symbol: CBX1
• Gene ID (NCBI): 10951
• RRID: AB_3670922
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P83916
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

One function of heterochromatin is the epigenetic silencing by sequestration of genes into transcriptionally repressed nuclear neighborhoods. Heterochromatin protein 1 (HP1) is a major component of heterochromatin and is a candidate for establishing and mediating gene silencing. This protein is expressed in all adult and embryonic tissues, as a homodimer.