CBX1 Recombinant antibody

CBX1 Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 83248-3-RR
Clone No.240075E1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), ELISA

HP1Hs beta, HP1 beta, Heterochromatin protein p25, Chromobox protein homolog 1, CBX

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inCHO cells, PC-12 cells, MCF-7 cells, NIH/3T3 cells, mouse spleen tissue, mouse brain tissue
Positive IF/ICC detected inHeLa cells, A431 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83248-3-RR targets CBX1 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CBX1 fusion protein Ag34901 Predict reactive species
Full Name chromobox homolog 1 (HP1 beta homolog Drosophila )
Calculated Molecular Weight 27 kDa
Observed Molecular Weight27 kDa
GenBank Accession NumberBC002609
Gene Symbol CBX1
Gene ID (NCBI) 10951
RRIDAB_3670922
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP83916
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

One function of heterochromatin is the epigenetic silencing by sequestration of genes into transcriptionally repressed nuclear neighborhoods. Heterochromatin protein 1 (HP1) is a major component of heterochromatin and is a candidate for establishing and mediating gene silencing. This protein is expressed in all adult and embryonic tissues, as a homodimer.

Protocols

Product Specific Protocols
WB protocol for CBX1 antibody 83248-3-RRDownload protocol
IF protocol for CBX1 antibody 83248-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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