CBX1 Recombinant antibody, PBS Only

CBX1 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

rat, mouse, human

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240075E1

Cat no : 83248-3-PBS

Synonyms

HP1Hs beta, HP1 beta, Heterochromatin protein p25, Chromobox protein homolog 1, CBX

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Validation Data Gallery

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  WB analysis using 83248-3-RR (same clone as 83248-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83248-3-RR (CBX1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83248-3-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 83248-3-RR (same clone as 83248-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CBX1 antibody (83248-3-RR, Clone: 240075E1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83248-3-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 83248-3-RR (same clone as 83248-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CBX1 antibody (83248-3-RR, Clone: 240075E1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83248-3-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 83248-3-RR (same clone as 83248-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CBX1 antibody (83248-3-RR, Clone: 240075E1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83248-3-PBS in a different storage buffer formulation.

• 

  IF Staining of A431 using 83248-3-RR (same clone as 83248-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CBX1 antibody (83248-3-RR, Clone: 240075E1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83248-3-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 83248-3-RR (same clone as 83248-3-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug CBX1 Recombinant antibody (83248-3-RR, Clone:240075E1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83248-3-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83248-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83248-3-RR against Human CBX1 were performed. The affinity constant is 3.31 nM.

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Product Information

The immunogen of 83248-3-PBS is CBX1 Fusion Protein expressed in E. coli.

• Tested Reactivity: rat, mouse, human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CBX1 fusion protein Ag34901
• Full Name: chromobox homolog 1 (HP1 beta homolog Drosophila )
• Calculated Molecular Weight: 27 kDa
• GenBank Accession Number: BC002609
• Gene Symbol: CBX1
• Gene ID (NCBI): 10951
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.