CoraLite®594-conjugated CAPRIN1 Monoclonal antibody

CAPRIN1 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

6B10F11

Cat no : CL594-66352

Synonyms

GPI-anchored membrane protein 1, Cytoplasmic activation- and proliferation-associated protein 1, Cell cycle-associated protein 1, Caprin-1, caprin 1

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Validation Data Gallery

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  IF Staining of HeLa using CL594-66352

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®594-conjugated CAPRIN1 antibody (CL594-66352, Clone: 6B10F11 ) at dilution of 1:100.

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  IF Staining of HeLa using CL594-66352

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®594 CAPRIN1 antibody (CL594-66352, Clone: 6B10F11 ) at dilution of 1:800.

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  IF Staining of HeLa using CL594-66352

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CL594-66352 (CAPRIN1 antibody) at dilution of 1:100.

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  FC experiment of NIH/3T3 using CL594-66352

  1X10^6 NIH/3T3 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human CAPRIN1 (CL594-66352, Clone:6B10F11) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL594-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: NIH/3T3 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66352 targets CAPRIN1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CAPRIN1 fusion protein Ag7413
• Full Name: cell cycle associated protein 1
• Calculated Molecular Weight: 78 kDa
• Observed Molecular Weight: 116 kDa
• GenBank Accession Number: BC001731
• Gene Symbol: CAPRIN1
• Gene ID (NCBI): 4076
• RRID: AB_2883566
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CAPRIN1(Caprin-1) is a cytoplasmic phosphoprotein which may regulate the transport and translation of mRNAs of proteins involved in synaptic plasticity in neurons and cell proliferation and migration in multiple cell types. Its levels, except in the brain, are tightly correlated with cellular proliferation. Suppression of expression of human Caprin-1 resulted in slowing of the proliferation rate,which suggests its essential for normal cellular proliferation.

Related Products

Flow Cytometry Perm Buffer (10X)

PF00011-C